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Analysis of Terpenelactones in Ginkgo biloba by High Performance Liquid Chromatography and Evaporative Light Scattering Detection
2001 Volume 49 Issue 9 Pages 1170-1173
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2001 Volume 49 Issue 9 Pages 1170-1173
A reversed phase HPLC method permitting the determination of 5 terpenelactones in Ginkgo biloba, without the need of any sample preparation is presented in this paper. The compounds were successfully separated within 25 min by using a C-12 column, an evaporative light scattering (ELS) detector and a mobile phase comprising of ammonium acetate buffer, methanol and isobutanol. All terpenelactones were detectable at concentrations as low as 20.3 μg/ml. The analysis of G. biloba market products showed remarkable variations in the lactone content, and more than 2 fold differences in the suggested daily doses of the total lactones, from 8.84 mg to 18.28 mg, respectively.
