The Preparation of Mitomycin C, Adriamycin and Daunomycin Covalently Bound to Antibodies as Improved Cancer Chemotherapeutic Agents

TOSHIO SUZUKI; ETSUKO SATO; KOZO GOTO; YUKO KATSURADA; KATSUO UNNO; TOSHIO TAKAHASHI

doi:10.1248/cpb.29.844

TOSHIO SUZUKI, ETSUKO SATO, KOZO GOTO, YUKO KATSURADA, KATSUO UNNO, TOSHIO TAKAHASHI

Author information

Keywords: cancer chemotherapy

JOURNAL FREE ACCESS

1981 Volume 29 Issue 3 Pages 844-848

DOI https://doi.org/10.1248/cpb.29.844

Details

Abstract

Mitomycin C, daunomycin and adriamycin, three potent cancer chemotherapeutic agents, were linked to immunoglobulins by the cyanogen bromide method and the conjugates were separated on Sepharose 6B. The most suitable conditions for binding of the drug to the antibody, while retaining both antibody and drug activity, included the use of 25-50 mol of cyanogen bromide per mol of IgG at pH 11 for the activation of immunoglobulin. The extent of substitution in this preparation was about 1 mol of drug per mol of IgG. This conjugate might be considered as a sustained release form of the antitumor agent.

Corresponding author

Register with J-STAGE for free!