1964 Volume 12 Issue 5 Pages 582-588
A new colorimetric determination of cholesterol has been established, using anhydrous aluminum chloride and anisaldehyde as reagents. The limit and scope of the reaction on steroid was investigated, and it is concluded that a steroid having a double bond not conjugated to a carbonyl group, or having a hydroxyl group easily dehydrated gives a positive result. Availability of the reagents and difference from other known color reactions are also discussed.