Effect of Chondroitinase on Dermatan Sulfate-Facilitated Arginine Amidase Released from Rabbit Ear Artery

Reiko Ishii-Nozawa; Tatsuya Naito; Mitsuo Mita; Kyosuke Miyazaki; Yoshifumi Matsuda; Koichi Takeuchi

doi:10.1248/bpb.33.150

Notes

Effect of Chondroitinase on Dermatan Sulfate-Facilitated Arginine Amidase Released from Rabbit Ear Artery

Reiko Ishii-Nozawa, Tatsuya Naito, Mitsuo Mita, Kyosuke Miyazaki, Yoshifumi Matsuda, Koichi Takeuchi

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Keywords: arginine amidase, dermatan sulfate, rabbit ear artery, chondroitinase ABC, chondroitinase ACII

JOURNAL FREE ACCESS

2010 Volume 33 Issue 1 Pages 150-152

DOI https://doi.org/10.1248/bpb.33.150

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Abstract

We examined the effects of chondroitinases on the release of dermatan sulfate (DS)-induced arginine amidase (AA) from rabbit ear artery. DS-induced AA release was significantly decreased by treatment with chondroitinase ABC (ABCase) in the rabbit ear artery. On the other hand, Chondroitinase ACII (ACIIase) enhanced spontaneous and DS-induced AA release. Heat-inactivated ABCase and ACIIase did not affect spontaneous and DS-induced AA release. Furthermore, ABCase, but not ACIIase and heat-inactivated chondroitinases, degraded DS. These results indicate that the facilitatory effect of DS-induced AA release from the rabbit ear artery is affected by the molecular size of DS.

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