Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Inhibitory Effects of Nardostachin on Nitric Oxide, Prostaglandin E2, and Tumor Necrosis Factor-α Production in Lipopolysaccharide Activated Macrophages
Hye Kyung Ju, Suk-Hwan Baek, Ren-Bo An, KiHwan Bae, Kun Ho Son, Hyun Pyo Kim, Sam Sik Kang, Sung Ho Lee, Jong Keun Son, Hyeun Wook Chang
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Keywords: Patrinia saniculaefolia, nardostachin, niric oxide, TNF-α, prostaglandin E2, cyclooxygenase-2
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2003 Volume 26 Issue 10 Pages 1375-1378

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Abstract

Nardostachin, which is an iridoid isolated from Patrinia saniculaefolia, was examined by assessing its effect on the production of tumor necrosis factor-α (TNF-α) and expression of 2 enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in lipopolysaccharide (LPS)-stimulated Raw264.7 macrophages. This compound consistently inhibited the production of nitric oxide (NO) and TNF-α production in a dose-dependent manner, with respective IC50 values of 12.3 and 16.2 μM. The decrease in quantity of NO products was accompanied by a decrease in the iNOS protein level, as assessed by Western blotting probed with specific anti-iNOS antibodies. In addition, this compound also reduced the COX-2 protein expression level and the attendant PGE2 production in LPS-stimulated macrophages. These results suggest that nardostachin may be useful for inhibiting the production of inflammatory mediators such as TNF-α, NO and PGE2 in inflammatory diseases.

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© 2003 The Pharmaceutical Society of Japan
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