Along with chrysophanol glucoside (I), mp 245-246°, C₂₁H₂₀O₉·1/2H₂O, and 8-O-β-D-glucopyranosides of physcion, emodin, aloe-emodin and rhein, three new anthraquinone glucosides, II : mp 187-189°, C₂₁H₂₀O₁₀·2H₂O, III : mp 239-241°, C₂₁H₂₀O₁₀·1/2H₂O and IV : mp 210-215°were isolated from the rhubarb, and II and III were respectively identified with 1, 8-dihydroxy-3 [-O-β-D-glucopyranosylmethyl]-aloe-emodin and 1-O-β-D-glucopyranosyl-emodin by spectral analyses of them, their permethylates and aglycones of permethylates. Although physical constants of I were quite the same with those of chrysophanein (1-O-β-D-glucopyranosyl-chrysophanol), I was found to be a mixture of 1-and 8-O-β-D-glucopyranosyl-chrysophanols by nuclear magnetic resonance spectral analysis of the aglycone of its permethylate.