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Association of Intra-tumoral Infiltrating Macrophages and Regulatory T Cells Is an Independent Prognostic Factor in Gastric Cancer after Radical Resection

  • Gastrointestinal Oncology
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

Macrophages (Mφ) and regulatory T cells (Tregs) are the major components of the inflammatory infiltrate in virtually all tumors. The objective of this study was to investigate the prognostic significance of Mφ and Tregs infiltration in advanced gastric cancer after radical resection.

Methods

CD68+ Mφ and FOXP3+ Tregs were assessed by immunohistochemistry in tissues from 107 patients with surgically advanced gastric cancer. The microlocalization of Mφ and Tregs cells with respect to the development of gastric cancer were given special concern. Prognostic value of normal, peritumoral, and intratumoral Mφ and Tregs densities was evaluated by Kaplan–Meier analysis and Cox regression.

Results

The results showed that the presence of intratumoral CD68+ Mφ was an independent prognostic factor for overall survival (OS) (P = 0.02). Moreover, the combination of high numbers of intratumoral CD68+ Mφ and FOXP3+ Tregs was associated with improved survival (P = 0.041). Five-year OS rate was only 27% for patients with low intratumoral Mφ and intratumoral Tregs compared with 62% for patients with high intratumoral Mφ and intratumoral Tregs. In addition, advanced intestinal-type gastric cancers were more likely to have fewer infiltrating Mφ than diffuse-type cancers (P = 0.024).

Conclusions

Association of intratumoral Mφ and Tregs is a promising independent predictor for survival in advanced gastric cancer. The results suggested that a combination of concomitant stimulation of intratumoral Mφ and Tregs may be an effective strategy for treatment of patients with advanced gastric cancer after radical resection.

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Acknowledgment

This study was supported by National Natural Science Foundation of China (No. 30801095) and the “863” program (2006AA02A402).

Disclosure

No potential conflicts of interest were disclosed.

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, Sun Yat-sen (Zhongshan) University, Guangzhou, People’s Republic of China

    Bo Wang PhD, Limin Zheng MD & Lian Li PhD

  2. State Key Laboratory of Oncology in South China, Sun Yat-sen (Zhongshan) University, Guangzhou, People’s Republic of China

    Dazhi Xu MD, Xingjuan Yu MD, Tong Ding PhD, Youqing Zhan MD & Limin Zheng MD

  3. Department of Abdominal Surgery, Cancer Center, Sun Yat-sen (Zhongshan) University, Guangzhou, People’s Republic of China

    Dazhi Xu MD & Youqing Zhan MD

  4. Department of Pathology, Cancer Center, Sun Yat-sen (Zhongshan) University, Guangzhou, People’s Republic of China

    Huilan Rao MD

  5. Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, Guangzhou, People’s Republic of China

    Lian Li PhD

Authors
  1. Bo Wang PhD
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  2. Dazhi Xu MD
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  3. Xingjuan Yu MD
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  4. Tong Ding PhD
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  6. Youqing Zhan MD
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  7. Limin Zheng MD
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Corresponding author

Correspondence to Lian Li PhD.

Additional information

Bo Wang and Dazhi Xu are contributed equally to this work.

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Wang, B., Xu, D., Yu, X. et al. Association of Intra-tumoral Infiltrating Macrophages and Regulatory T Cells Is an Independent Prognostic Factor in Gastric Cancer after Radical Resection. Ann Surg Oncol 18, 2585–2593 (2011). https://doi.org/10.1245/s10434-011-1609-3

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  • DOI: https://doi.org/10.1245/s10434-011-1609-3

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