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First published online 30 October 2007
doi: 10.1242/jcs.019729

Journal of Cell Science 120, 4035-4049 (2007)
Published by The Company of Biologists 2007
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Research Article

NCAM is ubiquitylated, endocytosed and recycled in neurons

Simone Diestel1,*, Daniel Schaefer1, Harold Cremer2,{ddagger} and Brigitte Schmitz1,{ddagger},§

1 Institute of Animal Sciences, Department of Biochemistry, University of Bonn, Katzenburgweg 9a, 53115 Bonn, Germany
2 Institut de Biologie du Développement de Marseille-Luminy, UMR 6216, CNRS/Université de la Méditeranée, Campus de Luminy-case 907, 13288 Marseille cedex 9, France

§ Author for correspondence (e-mail: schmitz{at}uni-bonn.de)

Accepted 12 September 2007

The neural cell adhesion molecule NCAM plays an important role during neural development and in the adult brain. To study the intracellular trafficking of NCAM in neurons, two major isoforms, NCAM140 or NCAM180, were expressed in primary cortical neurons and in the rat B35 neuroblastoma cell line. NCAM was endocytosed and subsequently recycled to the plasma membrane, whereas only a minor fraction was degraded in lysosomes. In cortical neurons, endocytosis of NCAM was detected in the soma, neurites and growth cones in a developmentally regulated fashion. Furthermore, we found that NCAM is mono-ubiquitylated at the plasma membrane and endocytosis was significantly increased in cells overexpressing ubiquitin. Therefore, we propose that ubiquitylation represents an endocytosis signal for NCAM.

Key words: Neural cell adhesion molecule NCAM, Intracellular trafficking, Endocytosis, Recycling, Ubiquitylation, Primary neurons




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