| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLES |
Department of Anatomy and Cell Biology (A.F., L.C.K., Y.G., G.T.), SUNY Health Science Center at Brooklyn, Brooklyn, New York 11203; Department of Cell Biology (C.V.E.W.), Vanderbilt University Medical Center, Nashville, Tennessee 37232; and the Department of Molecular Physiology and Biophysics (R.S.), Vanderbilt University Medical Center, Nashville, Tennessee 37232
Address all correspondence and requests for reprints to: Dr. Gladys Teitelman, Department of Anatomy and Cell Biology, SUNY Health Science Center at Brooklyn, Brooklyn, New York 11203.
The ability of the adult pancreas to generate new insulin (ß) cells has been controversial because of difficulties in unequivocally identifying the precursor population. We recently determined that ß cells were generated during development from precursors that expressed the homeodomain-containing transcription factor pancreas duodenum homeobox gene-1 (PDX-1). To investigate whether PDX-1+ stem cells are present in adult pancreas, we examined two animal models of diabetes. One model was produced by injecting adult mice with streptozotocin (SZ), a toxin that produces hyperglycemia due to rapid and massive ß cell death. After SZ-mediated elimination of existing IN+/PDX-1+ cells, a population of somatostatin (SOM)+/PDX-1+ cells, a cell type thought to represent an embryonic islet precursor cell, appeared in islets. The appearance of SOM+/PDX-1+ cells was followed in time by the differentiation to SOM+/IN+/PDX-1+cells. SOM+/PDX-1+ cells also appeared in islets of nonobese diabetic mice, a strain of mice in which ß cell destruction is immune-mediated. Our findings establish the existence of PDX-1+ ß cell precursors in the adult pancreas and indicate that their differentiation is induced by islet injury .
This article has been cited by other articles:
 |
|
 |
|
  Y. Sun, L. Zhang, H. F. Gu, W. Han, M. Ren, F. Wang, B. Gong, L. Wang, H. Guo, W. Xin, et al. Peroxisome Proliferator-Activated Receptor-{alpha} Regulates the Expression of Pancreatic/Duodenal Homeobox-1 in Rat Insulinoma (INS-1) Cells and Ameliorates Glucose-Induced Insulin Secretion Impaired by Palmitate Endocrinology, February 1, 2008; 149(2): 662 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kodama, F. Takeshita, S. Kanegasaki, T. Ochiya, and G. Quinn Pancreatic Endocrine and Exocrine Cell Ontogeny From Renal Capsule transplanted Embryonic Stem Cells in Streptozocin-injured Mice J. Histochem. Cytochem., January 1, 2008; 56(1): 33 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hanley and L. Rosenberg Transforming Growth Factor {beta} Is a Critical Regulator of Adult Human Islet Plasticity Mol. Endocrinol., June 1, 2007; 21(6): 1467 - 1477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Yin, J. Tao, D. D. Lee, J. Shen, M. Hara, J. Lopez, A. Kuznetsov, L. H. Philipson, and A. S. Chong Recovery of Islet {beta}-Cell Function in Streptozotocin- Induced Diabetic Mice: An Indirect Role for the Spleen Diabetes, December 1, 2006; 55(12): 3256 - 3263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Datar, D. S. Suryavanshi, and R. R. Bhonde Chick Pancreatic B Islets as an Alternative In Vitro Model for Screening Insulin Secretagogues Poult. Sci., December 1, 2006; 85(12): 2260 - 2264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Hussain, D. L. Porras, M. H. Rowe, J. R. West, W.-J. Song, W. E. Schreiber, and F. E. Wondisford Increased Pancreatic {beta}-Cell Proliferation Mediated by CREB Binding Protein Gene Activation Mol. Cell. Biol., October 15, 2006; 26(20): 7747 - 7759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Oyama, K. Minami, K. Ishizaki, M. Fuse, T. Miki, and S. Seino Spontaneous Recovery From Hyperglycemia by Regeneration of Pancreatic {beta}-Cells in Kir6.2G132S Transgenic Mice. Diabetes, July 1, 2006; 55(7): 1930 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Thyssen, E. Arany, and D. J. Hill Ontogeny of Regeneration of {beta}-Cells in the Neonatal Rat after Treatment with Streptozotocin Endocrinology, May 1, 2006; 147(5): 2346 - 2356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Lu, Z. Wang, and M. Zhu Human bone marrow mesenchymal stem cells transfected with human insulin genes can secrete insulin stably. Ann. Clin. Lab. Sci., March 1, 2006; 36(2): 127 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suzuki, H. Nakauchi, and H. Taniguchi Prospective Isolation of Multipotent Pancreatic Progenitors Using Flow-Cytometric Cell Sorting Diabetes, August 1, 2004; 53(8): 2143 - 2152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li, Z. Yi, M. Seno, and I. Kojima Activin A and Betacellulin: Effect on Regeneration of Pancreatic {beta}-Cells in Neonatal Streptozotocin-Treated Rats Diabetes, March 1, 2004; 53(3): 608 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lechner, Y.-G. Yang, R. A. Blacken, L. Wang, A. L. Nolan, and J. F. Habener No Evidence for Significant Transdifferentiation of Bone Marrow Into Pancreatic {beta}-Cells In Vivo Diabetes, March 1, 2004; 53(3): 616 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kuroda, Y. Yamasaki, and A. Imagawa {beta}-Cell Regeneration in a Patient with Type 1 Diabetes Mellitus Who Was Receiving Immunosuppressive Therapy Ann Intern Med, November 18, 2003; 139(10): W-81 - W-81. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Li, M. Seno, H. Yamada, and I. Kojima Betacellulin improves glucose metabolism by promoting conversion of intraislet precursor cells to {beta}-cells in streptozotocin-treated mice Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E577 - E583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ber, K. Shternhall, S. Perl, Z. Ohanuna, I. Goldberg, I. Barshack, L. Benvenisti-Zarum, I. Meivar-Levy, and S. Ferber Functional, Persistent, and Extended Liver to Pancreas Transdifferentiation J. Biol. Chem., August 22, 2003; 278(34): 31950 - 31957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Duvillie, M. Attali, V. Aiello, E. Quemeneur, and R. Scharfmann Label-Retaining Cells in the Rat Pancreas: Location and Differentiation Potential in Vitro Diabetes, August 1, 2003; 52(8): 2035 - 2042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Paris, C. Bernard-Kargar, M.-F. Berthault, L. Bouwens, and A. Ktorza Specific and Combined Effects of Insulin and Glucose on Functional Pancreatic {beta}-Cell Mass in Vivo in Adult Rats Endocrinology, June 1, 2003; 144(6): 2717 - 2727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gu, J. Dubauskaite, and D. A. Melton Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors Development, March 7, 2003; 129(10): 2447 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Pospisilik, J. Martin, T. Doty, J. A. Ehses, N. Pamir, F. C. Lynn, S. Piteau, H.-U. Demuth, C. H.S. McIntosh, and R. A. Pederson Dipeptidyl Peptidase IV Inhibitor Treatment Stimulates {beta}-Cell Survival and Islet Neogenesis in Streptozotocin-Induced Diabetic Rats Diabetes, March 1, 2003; 52(3): 741 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lechner and J. F. Habener Stem/progenitor cells derived from adult tissues: potential for the treatment of diabetes mellitus Am J Physiol Endocrinol Metab, February 1, 2003; 284(2): E259 - E266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-Q. Zhang, H. Zhang, A. Maeshima, H. Kurihara, J.-i. Miyagawa, T. Takeuchi, and I. Kojima Up-Regulation of the Expression of Activins in the Pancreatic Duct by Reduction of the {beta}-Cell Mass Endocrinology, September 1, 2002; 143(9): 3540 - 3547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lipsett and D. T. Finegood {beta}-Cell Neogenesis During Prolonged Hyperglycemia in Rats Diabetes, June 1, 2002; 51(6): 1834 - 1841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-p. Huang, K. Chu, E. Nemoz-Gaillard, D. Elberg, and M.-J. Tsai Neogenesis of {beta}-Cells in Adult BETA2/NeuroD-Deficient Mice Mol. Endocrinol., March 1, 2002; 16(3): 541 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hall, C. M. Matter, X. Wang, and G. H. Gibbons Hyperglycemia Inhibits Vascular Smooth Muscle Cell Apoptosis Through a Protein Kinase C-Dependent Pathway Circ. Res., September 29, 2000; 87(7): 574 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Seijffers, O. Ben-David, Y. Cohen, A. Karasik, M. Berezin, C. B. Newgard, and S. Ferber Increase in PDX-1 Levels Suppresses Insulin Gene Expression in RIN 1046-38 Cells Endocrinology, July 1, 1999; 140(7): 3311 - 3317. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. JESNOWSKI, P. MULLER, W. SCHARECK, S. LIEBE, and M. LOHR Immortalized Pancreatic Duct Cells in vitro and in vivo Ann. N.Y. Acad. Sci., June 30, 1999; 880(1): 50 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Petrik, E. Arany, T. J. McDonald, and D. J. Hill Apoptosis in the Pancreatic Islet Cells of the Neonatal Rat Is Associated with a Reduced Expression of Insulin-Like Growth Factor II that May Act as a Survival Factor Endocrinology, June 1, 1998; 139(6): 2994 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Gross, L. Weiss, I. Reibstein, J. van den Brand, H. Okamoto, A. Clark, and S. Slavin Amelioration of Diabetes in Nonobese Diabetic Mice with Advanced Disease by Linomide-Induced Immunoregulation Combined with Reg Protein Treatment Endocrinology, May 1, 1998; 139(5): 2369 - 2374. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
