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绝对共轭共聚焦拉曼光谱技术研究

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文章导航 > 光电工程 > 2021 Vol. 48 > No. 6 > 200398

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尚林东，梁鹏，吴青宜，等. 绝对共轭共聚焦拉曼光谱技术研究[J]. 光电工程，2021，48(6): 200398. doi: 10.12086/oee.2021.200398
引用本文: 尚林东，梁鹏，吴青宜，等. 绝对共轭共聚焦拉曼光谱技术研究[J]. 光电工程，2021，48(6): 200398. doi: 10.12086/oee.2021.200398
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Shang L D, Liang P, Wu Q Y, et al. Research on absolute conjugation confocal Raman spectroscopy technology[J]. Opto-Electron Eng, 2021, 48(6): 200398. doi: 10.12086/oee.2021.200398
Citation: Shang L D, Liang P, Wu Q Y, et al. Research on absolute conjugation confocal Raman spectroscopy technology[J]. Opto-Electron Eng, 2021, 48(6): 200398. doi: 10.12086/oee.2021.200398
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绝对共轭共聚焦拉曼光谱技术研究

    • 1.

      中国科学院长春光学精密机械与物理研究所，吉林 长春 130031

    • 2.

      中国科学院大学，北京 100049

    • 3.

      长光辰英生物科学仪器有限公司，吉林 长春 130031

  • 基金项目:
    国家重点研发计划“政府间国际科技创新合作/港澳台科技创新合作”重点专项(SQ2018YFE010810)
详细信息
    作者简介:
  • 尚林东(1998-)，男，博士研究生，主要从事时间门控拉曼光谱的研究。E-mail：shanglindong20@mails.ucas.ac.cn
    • 通讯作者: 李备(1983-)，男，博士，研究员，主要从事单细胞分选、拉曼光谱的研究。E-mail：beili@ciomp.ac.cn

  • 中图分类号: TN253;O734

Research on absolute conjugation confocal Raman spectroscopy technology

    • 1.

      Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, Jilin 130031, China

    • 2.

      University of Chinese Academy of Sciences, Beijing 100049, China

    • 3.

      HOOKE Instruments Ltd, Changchun, Jilin 130031, China

  • Fund Project: National Key R & D Program "International Cooperation in Science and Technology Innovation Between Governments/Cooperation in Science and Technology Innovation Between Hong Kong, Macao and Taiwan" (SQ2018YFE010810)
More Information

    摘要

  • 为解决传统拉曼光谱信号强度弱、信噪比低的问题，本文提出一种新型的共聚焦拉曼系统，通过外接光子晶体光纤实现共聚焦点的绝对共轭，总结了光子晶体光纤耦合过程中出现的技术问题，并对实际样品进行测试。与Thorlabs、OZ两种常规共聚焦拉曼系统所用光纤、Witec 532 nm-alpha300R拉曼系统进行比较，在相同激光强度和积分时间下，本文信噪比为73.8382，显著高于Thorlabs、OZ两种光纤的37.1557和40.0342，而相较于Witec 532 nm-alpha300R的65.5312，也提升了12.68%，高质量的拉曼信号使得该绝对共轭共聚焦拉曼系统具有广阔的市场前景和超高的市场竞争力。

    To solve the problems of weak signal strength and low signal-to-noise ratio in traditional Raman spectroscopy, a new confocal Raman system is proposed in this paper. The absolute conjugation of the confocal point is realized by external photonic crystal fiber. The technical problems in the coupling process of photonic crystal fiber are summarized, and the actual samples are tested. Compared with conventional confocal Raman fibers such as Thorlabs and OZ and Witec 532 nm-alpha300R Raman system, the signal-to-noise ratio in this paper is 73.8382 at the same laser intensity and integration time, which is significantly higher than that of Thorlabs and OZ (37.1557 and 40.0342, respectively). Compared with the signal-to-noise ratio of 65.5312 for Witec 532 nm-alpha300R, it also increased by 12.68%. High-quality Raman signal makes the absolute conjugated confocal Raman system have broad market prospects and ultra-high market competitiveness.

    • Overview

  • Overview: After the discovery of the Raman scattering effect, due to its high sensitivity and non-invasiveness to test samples, it has been more and more used in materials testing, jewelry identification and other fields. However, in the direction of biological samples, such as bacterial metabolism detection, microbial discrimination, etc., the intensity of Raman spectroscopy is relatively weak, and the signal-to-noise ratio is low. As a conventional Raman signal acquisition method, the confocal Raman system occupies an important position in many Raman systems. However, most confocal Raman systems mostly use small holes or slits, and rarely use photonic crystal fibers. Aiming at the problems of weak signal strength and low signal-to-noise ratio of traditional Raman spectroscopy, a new confocal Raman system is proposed. The system realizes the absolute conjugation of the confocal point through the external photonic crystal fiber. Secondly, the difference in imaging accuracy between photonic crystal fiber and other single-mode fibers is verified, and it is found that the imaging accuracy of photonic crystal fiber is much higher than that of ordinary single-mode fiber. Then, the actual samples were tested and verified, and Escherichia coli with high background noise was screened out. The test results were compared with the optical fibers used in Thorlabs and OZ conventional confocal Raman systems and Witec 532 nm-alpha300R confocal Raman systems. Under the conditions of the same laser intensity of 3 mW and integration time of 5 s, the signal-to-noise ratio obtained is 73.8382, which is higher than that of Thorlabs and OZ systems. Compared with the 65.5312 of the Witec 532 nm-alpha300R confocal Raman system, the Raman signal quality of the two single-mode fibers are 37.1557 and 40.0342 respectively, an increase of 12.68%. It can be seen that the quality of the Raman signal obtained in this paper is relatively high. Absolutely conjugated confocal Raman system will promote the application of photonic crystal fiber in biological cell Raman, and has a very broad application prospect.

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  • 图 1  绝对共轭共聚焦拉曼光路图

    Figure 1.  Absolute conjugated confocal Raman spectrogram

    下载: 全尺寸图片 幻灯片

    图 2  光子晶体光纤结构图

    Figure 2.  Structure diagram of the photonic crystal fiber

    下载: 全尺寸图片 幻灯片

    图 3  实验硅片处理前(a)和后(b)对比图

    Figure 3.  Comparison of the experimental silicon before (a) and after (b) treatment

    下载: 全尺寸图片 幻灯片

    图 4  本文采集样品图谱

    Figure 4.  Raman spectrum of the collected samples

    下载: 全尺寸图片 幻灯片

    图 5  数据处理流程图

    Figure 5.  Flow chart of data processing

    下载: 全尺寸图片 幻灯片

    图 6  光子晶体光纤与普通单模光纤成像精度对比图

    Figure 6.  Comparison of imaging accuracy between the photonic crystal fiber and the ordinary single-mode fiber

    下载: 全尺寸图片 幻灯片

    图 7  两种光纤和Witec与本文采集大肠杆菌拉曼信号对比图

    Figure 7.  Comparison of Raman signals of Escherichia coli collected by two kinds of optical fibers and Witec and this article

    下载: 全尺寸图片 幻灯片

    表 1  两种光纤和Witec与本文信噪比对比表

    Table 1.  SNR comparison table between Witec and this paper

    Thorlabs OZ Witec Our
    SNR 37.2384 40.0318 64.6236 70.5219
    36.8945 40.2175 66.1375 71.3961
    37.3341 39.8532 65.8325 73.5965
    Average 37.1557 40.0342 65.5312 73.8382
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出版历程
收稿日期:  2020-10-26
修回日期:  2021-04-01
刊出日期:  2021-06-01

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