ABSTRACT
The objective of this study was to investigate the obtaining of antioxidant bioactive extracts from the Ataulfo mango seed. The influence of two drying methods (convection and lyophilization) and four extraction techniques (water bath, ultrasound, microwave, and enzymatic) were investigated for their total polyphenol content and antioxidant activity (DPPH and ABTS). The HPLC–MS analysis was also developed to identify the compounds. The results showed that lyophilization drying and ultrasound extraction are the most efficient methods with a phenolic content of 29.45 mg GAE/g and a potent antioxidant activity of 97.47% (DPPH) and 289.87 mg TE/g (ABTS) that was higher than that reported in fruits that are a good source of antioxidants. Enzyme-assisted extraction (EAE) revealed a high polyphenol content and ABTS antioxidant activity, although DPPH antioxidant activity was lower than ultrasound and microwave. This method is proposed as an interesting field of research. HPLC–MS analysis revealed the presence of 16 compounds belonging to the families of lignans, gallotannins, phenolic 106acids, and flavonols. Our results demonstrate that with the right technology bioactive extracts with a powerful antioxidant activity from by-products of mango processing can be obtained. The extracts have multiple applications in the food and pharmaceutical industry.
