Due to richness in essential amino acids, phospholipids, astaxanthin and omega-3 polyunsaturated fatty acids (PUFAs), Antarctic krill (Euphausia superba) which was abundant and had high nutritional value was considered to have great development value. At present, the analysis of fatty acid contents in Antarctic krill and its products mainly focused on the determination of total fatty acid contents. Owing to the effect of bioavailability for PUFAs in different esters, it was necessary to accurately quantify the content of fatty acid in different types of esters in Antarctic krill and its products. In order to clarify the content of phospholipids (PL) fatty acids in Antarctic krill oil, a gas chromatography analysis method for the content of fatty acids in PL in Antarctic krill oil was established based on solid phase extraction. After optimizing the various conditions of the impact analysis, under the optimal conditions, the recoveries of the standard addition measured by this method were between 84.22%-94.45%, and the intra-day and inter-day precision were between 0.79%-6.09%. This method was applied to measure the changes in the content of various PL fatty acids of Antarctic krill oil during storage. The results showed that the content of PL fatty acids decreased during the 21-day storage process, and the degradation rate increased with increasing temperature. The present study provided methodological support for the study of lipids in Antarctic krill.