关键词: 组织培养, 组织培养, 褐化, PPO基因, PAL基因

Abstract: In order to clear the composition of pathogens of wheat basal rot with symptoms similar to wheat sharp eyespot, screening the effective efficacy, and provide theoretical basis for design effective prevention measures. [Method] Using the conventional isolation method, we get Rhizoctonia, Fusarium and Bipolaris isolates from 60 wheat samples in different areas and determined the pathogenicity. We measured indoor biological activities of 4 fungicides such as sedaxane to Rhizootonia cerealis and Fusarium asiaticum using by the mycelium growth rate method and validated the efficacies by field trails.[Result] The result indicated that Rhizoctonia, Fusarium and Bipolaris all can infect wheat, and the typical symptoms in wheat stem base were similar to wheat sharp eyespot. The drug efficacy comparison tests of sedaxane and the pesticide mixture with fludioxonil or other drugs were carried on in the field, and the results showed that the effect of mixed matches was higher than the single dosage obviously. The effective median dos (EC₅₀) of sedaxane fungicides againstＲhizootonia cerealis were 0.099 mg/L, and the EC₅₀ of sedaxane fungicides against Fusarium asiaticum were 126.974 mg/L.[Conclusion] The wheat basal rot diseases with symptoms similar to wheat sharp eyespot were complex diseases caused by multi-species pathogens such as Rhizoctonia, Fusarium and Bipolaris.When preventing and controlling the diseases, the composite effects to Rhizoctonia, Fusarium, Bipolaris and other pathogens should be considered.