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Published online before print May 31, 2007

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(Journal of Leukocyte Biology. 2007;82:603-607.)
© 2007 by Society for Leukocyte Biology

Differential regulation of CD11b on T cells and monocytes in response to unripe apple polyphenols

Veterinary Molecular Biology, Montana State University, Bozeman, Montana, USA

¹ Correspondence: Veterinary Molecular Biology, Montana State University, 960 Technology Blvd., Bozeman, MT 59718, USA. E-mail: uvsmj{at}montana.edu

ABSTRACT

Leukocyte adhesion and migration are mediated partially by CD11b/CD18 (membrane-activated complex-1, CR3). Earlier studies have demonstrated a role for green tea polyphenols in down-regulating CD11b on CD8⁺ T cells and monocytes. We have shown recently a stimulatory effect of unripe apple polyphenols (APP) on T cells. Thus, we compared the effect of APP on bovine T cell and monocyte CD11b expression. Purified bovine monocytes and monocyte-depleted PBLs were cultured with APP. CD11b levels decreased on monocytes in response to APP. In contrast, a T cell subset responded to APP by up-regulating CD11b. The CD11b regulation was not seen on T cells or monocytes treated with APP fractions depleted of tannins. The APP-induced down-regulation of CD11b on monocytes was inhibited by an anti-CD11b mAb, consistent with previous studies showing that polyphenols bind CD11b. As expected, the anti-CD11b mAb had no effect on the APP response in resting T cells, as these cells lacked CD11b. Consistent with the changes in surface CD11b expression, APP-treated T cells showed increased adherence to plastic, whereas monocyte adhesion was reduced. APP also induced cytokine gene expression in T cells. Some polyphenols are thought of as anti-inflammatory agents; however, these data, as well as other ongoing studies, indicate they have a proinflammatory effect on T cells. In vivo, plant polyphenols may enhance T cell migration and function at sites of inflammation, where they could induce rapid, immune-regulatory and innate-like immune responses.

Key Words: CR3 • MAC-1 • tannin

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