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Blood, 1 October 2006, Vol. 108, No. 7, pp. 2339-2348. Prepublished online as a Blood First Edition Paper on June 22, 2006; DOI 10.1182/blood-2006-02-004291. This Article Full Text Full Text (PDF) Supplemental Methods and Figure All Versions of this Article: blood-2006-02-004291v1 108/7/2339    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by van Beijnum, J. R. Articles by Griffioen, A. W. Search for Related Content PubMed PubMed Citation Articles by van Beijnum, J. R. Articles by Griffioen, A. W. Related Collections Hemostasis, Thrombosis, and Vascular Biology Neoplasia Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Gene expression of tumor angiogenesis dissected: specific targeting of colon cancer angiogenic vasculature Judy R. van Beijnum, Ruud P. Dings, Edith van der Linden, Bernadette M. M. Zwaans, Frans C. S. Ramaekers, Kevin H. Mayo, and Arjan W. Griffioen From the Departments of Pathology and Molecular Cell Biology, Angiogenesis Laboratory, Research Institute for Growth and Development (GROW), Maastricht University, Maastricht, The Netherlands; and the Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN. Crucial to designing angiostatic and vascular targeting agents is the identification of target molecules. Because angiogenesis is not limited to pathologic conditions, careful evaluation of putative therapeutic targets is warranted to prevent adverse effects associated with impaired physiologic angiogenesis. To identify tumor-specific angiogenesis markers, we compared transcriptional profiles of angiogenic endothelial cells isolated from malignant and nonmalignant tissues with those of resting endothelial cells. We identified 17 genes that showed specific overexpression in tumor endothelium but not in angiogenic endothelium of normal tissues, creating a therapeutic window for tumor vasculature-specific targeting. Antibody targeting of 4 cell-surface–expressed or secreted products (vimentin, CD59, HMGB1, IGFBP7) inhibited angiogenesis in vitro and in vivo. Finally, targeting endothelial vimentin in a mouse tumor model significantly inhibited tumor growth and reduced microvessel density. Our results demonstrate the usefulness of the identification and subsequent targeting of specific tumor endothelial markers for anticancer therapy. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. S. Butler, R. A. Dean, E. M. Tam, and C. M. Overall Pharmacoproteomics of a Metalloproteinase Hydroxamate Inhibitor in Breast Cancer Cells: Dynamics of Membrane Type 1 Matrix Metalloproteinase-Mediated Membrane Protein Shedding Mol. Cell. Biol., August 1, 2008; 28(15): 4896 - 4914. [Abstract] [Full Text] [PDF] A. W. Griffioen New entry for angiostatic cancer treatment Blood, February 15, 2008; 111(4): 1755 - 1756. [Full Text] [PDF] V. L. Thijssen, S. Hulsmans, and A. W. Griffioen The Galectin Profile of the Endothelium: Altered Expression and Localization in Activated and Tumor Endothelial Cells Am. J. Pathol., February 1, 2008; 172(2): 545 - 553. [Abstract] [Full Text] [PDF] M. Mimeault and S. K. Batra Interplay of distinct growth factors during epithelial mesenchymal transition of cancer progenitor cells and molecular targeting as novel cancer therapies Ann. Onc., October 1, 2007; 18(10): 1605 - 1619. [Abstract] [Full Text] [PDF] D. M.E.I. Hellebrekers, V. Melotte, E. Vire, E. Langenkamp, G. Molema, F. Fuks, J. G. Herman, W. Van Criekinge, A. W. Griffioen, and M. van Engeland Identification of Epigenetically Silenced Genes in Tumor Endothelial Cells Cancer Res., May 1, 2007; 67(9): 4138 - 4148. [Abstract] [Full Text] [PDF]

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