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Progressive Evaluation of Apoptosis, Proliferation, and Angiogenesis in Fresh Rat Ovarian Autografts Under Remote Ischemic Preconditioning

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Abstract

This study evaluated the remote ischemic preconditioning (R-IPC) early and late repercussion on fresh ovarian transplants, aiming to assess a probable protective effect in ovarian follicular pool. Sixty Wistar EPM-1 rats were used, divided in 2 study groups: ovarian transplantation (Tx) and Tx + R-IPC, submitted to ovary transplant with or without R-IPC, respectively. These groups were subdivided according to the date for euthanasia: 4th, 7th, 14th, 21st, and 30th days of the postoperatory period. Morphology, morphometry, neoangiogenesis (vascular endothelial growth factor [VEGF]), proliferative activity (Ki-67), and apoptosis (cleaved caspase-3) were evaluated. Remote ischemic preconditioning was performed in the common iliac artery. Fresh autologous ovarian tissue was implanted integrally in the retroperitoneum. All animals showed resumption of estrous phase after ovary transplantation. Remote ischemic preconditioning attenuated the lesions progressively from the 7th day, with greater number of the immature follicles (14 days, P < .05), but didn’t affect mature follicles and corpora lutea (P > .05). Immunohistochemical analyzes, taken as a whole, show that R-IPC benefic effect is more evident in the later periods of evaluation, when a greater proliferative activity (14, 21, and 30 days, P < .05) and lesser cell apoptotic activity (21 and 30 days, P < .05). The VEGF expression was similar in all times (P > .05). Remote ischemic preconditioning could have a benefic effect in the progressive evaluation of freshly grafted ovarian, especially on the latest phases of the posttransplant period. The 14th day was a landmark in the recuperation of the graft. Further investigations are necessary to determine the role of R-IPC in this scenario and its effect in frozen-thawed ovarian tissue.

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Authors and Affiliations

  1. Gynecology Division, Laboratory of Structural and Molecular Gynecology (LIM-58), Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina da Universidade de São Paulo (FMUSP), Galvão Bueno St, 499. Bloco A. Apto31, 01506, Liberdade, São Paulo, Brasil

    Luciana Lamarão Damous & Edmund Chada Baracat

  2. Universidade da Cidade de São Paulo (Unicid), São Paulo, Brasil

    Sônia Maria da Silva

  3. Departamento de Morfologia e Genética, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brasil

    Adriana Aparecida Ferraz Carbonel & Manuel de Jesus Simões

  4. Laboratory of Surgical Physiopatology (LIM-62), Departamento de Cirurgia, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brasil

    Edna Frasson de Souza Montero

Authors
  1. Luciana Lamarão Damous
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  2. Sônia Maria da Silva
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  3. Adriana Aparecida Ferraz Carbonel
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  4. Manuel de Jesus Simões
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  5. Edmund Chada Baracat
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  6. Edna Frasson de Souza Montero
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Correspondence to Luciana Lamarão Damous.

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Damous, L.L., Silva, S.M.d., Carbonel, A.A.F. et al. Progressive Evaluation of Apoptosis, Proliferation, and Angiogenesis in Fresh Rat Ovarian Autografts Under Remote Ischemic Preconditioning. Reprod. Sci. 23, 803–811 (2016). https://doi.org/10.1177/1933719115620493

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