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Assisted Reproductive Technologies (ART) With Baboons Generate Live Offspring: A Nonhuman Primate Model for ART and Reproductive Sciences

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Abstract

Human reproduction has benefited significantly by investigating nonhuman primate (NHP) models, especially rhesus macaques. To expand the Old World monkey species available for human reproductive studies, we present protocols in baboons, our closest Old World primate relatives, for assisted reproductive technologies (ART) leading to live born offspring. Baboons complement rhesus by confirming or modifying observations generated in humans often obtained by the study of clinically discarded specimens donated by anonymous infertility patient couples. Here, baboon ART protocols, including oocyte collection, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), preimplantation development to blastocyst stage, and embryo transfer techniques are described. With baboon ART methodologies in place, motility during baboon fertilization was investigated by time-lapse video microscopy (TLVM). The first ART baboons produced by ICSI, a pair of male twins, were delivered naturally at 165 days postgestation. Genetic testing of these twins confirmed their ART parental origins and demonstrated that they are unrelated fraternal twins not identicals. These results have implications for ART outcomes, embryonic stem cell (ESC) derivation, and reproductive sciences.

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Authors and Affiliations

  1. Division of Developmental Regenerative Medicine, Department of Obstetrics, Gynecology, and Reproductive Sciences, Pittsburgh Development Center, Magee-Womens Research Institute University of Pittsburgh, Pittsburgh, PA, 15213, USA

    Calvin R. Simerly PhD, Carlos A. Castro MD, Laura Hewitson PhD & Gerald Schatten PhD

  2. Department of Cell Biology-Physiology, Pittsburgh Development Center, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, PA, USA

    Ethan Jacoby BS

  3. Department of Bioengineering, Pittsburgh Development Center, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, PA, USA

    Kevin Grund BS, Janet Turpin BS, Dave McFarland BS & Jamie Champagne BS

  4. Southwest National Primate Research Center (SNPRC), Southwest Foundation for Biomedical Research, San Antonio, TX, USA

    Joe B. Jimenez Jr BS, Pat Frost DVM & Cassondra Bauer DVM

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  1. Calvin R. Simerly PhD
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  2. Carlos A. Castro MD
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  3. Ethan Jacoby BS
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  9. Pat Frost DVM
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  10. Cassondra Bauer DVM
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  11. Laura Hewitson PhD
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  12. Gerald Schatten PhD
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Correspondence to Gerald Schatten PhD.

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Simerly, C.R., Castro, C.A., Jacoby, E. et al. Assisted Reproductive Technologies (ART) With Baboons Generate Live Offspring: A Nonhuman Primate Model for ART and Reproductive Sciences. Reprod. Sci. 17, 917–930 (2010). https://doi.org/10.1177/1933719110374114

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