Elsevier

SLAS Discovery

Volume 12, Issue 7, October 2007, Pages 999-1005
SLAS Discovery

Articles
Image-Based High-Throughput Quantification of Cellular Fat Accumulation

https://doi.org/10.1177/1087057107306502Get rights and content
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open access

A number of biochemical methods are available for measuring fat accumulation in cell culture. The authors report a simple image-based method for measuring fat accumulation in adipocytes using a combination of high-throughput brightfield microscopy and image analysis, which was validated biochemically using Oil-Red-O. The quickest and most accurate method of analysis was one based on thresholding brightfield images and determining the area of fat droplets per image. Thus, the authors have developed a simple high-throughput, label-free method for measuring fat accumulation that is applicable to any cell or tissue type where fat droplets are visible under light microscopy.

Key words

image analysis
lipids
high-content screening

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