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Follistatin Is Induced by IL-1β and TNF-α in Stromal Cells From Endometrioma

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Abstract

The aim of this study is to examine the regulation of follistatin, an activin-binding protein, in endometriosis. Endometrioma stromal cells (EoSCs) were obtained from 9 patients undergoing laparoscopy of the ovarian endometrioma. In cultured EoSCs, interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α), which could induce activin-A, also induced follistatin messenger RNA (mRNA) and protein. The cystic fluid of endometrioma from 8 patients was obtained to measure the concentration of activin-A and follistatin by enzyme-linked immunosorbent assay (ELISA). Also, activin activity in the fluid was examined by erythroid differentiation assay using mouse erythroleukemia F5-5.fl cells. In the cystic fluid of endometrioma, the mean values of activin-A and follistatin concentration were 36.8 ng/mL and 4.0 ng/mL, respectively. In a bioassay, all 8 samples exhibited activin activity, which was equivalent to recombinant activin-A activity of 12.8 ± 1.4 ng/mL. Although follistatin was present in the cystic fluid of endometrioma, the activity of activin, which is an exacerbation factor of endometriosis, was predominant in vivo.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan

    Ikumi Akiyama MD, Osamu Yoshino MD, PhD, Yutaka Osuga MD, PhD, Gentaro Izumi MD, Yoko Urata MD, Yasushi Hirota MD, PhD, Tetsuya Hirata MD, PhD, Miyuki Harada MD, PhD, Kaori Koga MD, PhD & Shiro Kozuma MD, PhD

  2. 6 Chemical Genomics Research Group, ASI, RIKEN, Saitama, Japan

    Kenji Ogawa PhD

Authors
  1. Ikumi Akiyama MD
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  2. Osamu Yoshino MD, PhD
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  3. Yutaka Osuga MD, PhD
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  4. Gentaro Izumi MD
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  5. Yoko Urata MD
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  6. Yasushi Hirota MD, PhD
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  7. Tetsuya Hirata MD, PhD
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  8. Miyuki Harada MD, PhD
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  9. Kaori Koga MD, PhD
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  10. Kenji Ogawa PhD
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  11. Shiro Kozuma MD, PhD
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Corresponding authors

Correspondence to Osamu Yoshino MD, PhD or Yutaka Osuga MD, PhD.

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Akiyama, I., Yoshino, O., Osuga, Y. et al. Follistatin Is Induced by IL-1β and TNF-α in Stromal Cells From Endometrioma. Reprod. Sci. 20, 675–679 (2013). https://doi.org/10.1177/1933719112463253

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