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Research Article Free access | 10.1172/JCI108992

Vagal, Cholinergic Regulation of Pancreatic Polypeptide Secretion

T. W. Schwartz, J. J. Holst, J. Fahrenkrug, S. Lindkær Jensen, O. V. Nielsen, J. F. Rehfeld, O. B. Schaffalitzky de Muckadell, and F. Stadil

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by Schwartz, T. in: JCI | PubMed | Google Scholar

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by Holst, J. in: JCI | PubMed | Google Scholar

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by Fahrenkrug, J. in: JCI | PubMed | Google Scholar

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by Jensen, S. in: JCI | PubMed | Google Scholar

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by Nielsen, O. in: JCI | PubMed | Google Scholar

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by Rehfeld, J. in: JCI | PubMed | Google Scholar

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by de Muckadell, O. in: JCI | PubMed | Google Scholar

Institute of Medical Biochemistry, University of Aarhus, Århus, Denmark

Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

Department of Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark

Find articles by Stadil, F. in: JCI | PubMed | Google Scholar

Published March 1, 1978 - More info

Published in Volume 61, Issue 3 on March 1, 1978
J Clin Invest. 1978;61(3):781–789. https://doi.org/10.1172/JCI108992.
© 1978 The American Society for Clinical Investigation
Published March 1, 1978 - Version history
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Abstract

The effect of efferent, parasympathetic stimulation upon pancreatic polypeptide (PP) secretion was studied in three ways: (a) Plasma PP concentrations increased in response to insulin-induced hypoglycemia in both normal subjects, from 11 pM (9.5-12.5) to 136 pM (118-147), n = 8 (median and interquartile range) and in duodenal ulcer patients, from 33 pM (21-52) to 213 pM (157-233), n = 7. The PP response to hypoglycemia was diminished by atropine in normal subjects (P < 0.005) and completely abolished by vagotomy in the duodenal ulcer patients. (b) Electrical stimulation, 8 Hz, of the vagal nerves in anesthetized pigs induced an increase in portal PP concentrations within 30 s from 32 pM (28-39) to 285 pM (248-294), n = 12. Minimal stimulatory frequency was 0.5 Hz and maximal stimulatory frequency 8-12 Hz. Atropine inhibited the PP response to electrical stimulation. Median inhibition with 0.5 mg of atropine/kg body wt was 74%, range 31-90%, n = 6. The response was eliminated by hexamethonium. Adrenergic alpha and beta blockade did not influence the release of PP in response to vagal stimulation. (c) Acetylcholine stimulated, in a dose-dependent manner, the secretion of PP from the isolated perfused porcine pancreas, half-maximal effective dose being 0.19 μM; maximal PP output in response to 5 min stimulation was 228 pmol, range 140-342 pmol, n = 5. Atropine completely abolished this response.

The results of the present study together with the previously demonstrated poor PP response to food in vagotomized patients, indicate that vagal, cholinergic stimulation is a major regulator of PP secretion.

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