Covalent Binding of Streptavidin on Gold Magnetic Nanoparticles for Bead Array Fabrication
In this study, we report the chemical synthesis and functionalization of streptavidin coated gold magnetic nanoparticles (GMNPs) and the immobilization of single-stranded biotinylated oligonucleotides onto these particles. By using covalent interaction or physical adsorption, two kinds
of streptavidin coated GMNPs (SA-GMNPs) were prepared. The quantity and stability of streptavidin bound to the GMNPs using different methods were determined by UV-Vis spectrometer. The results indicated that by physical absorption the GMNPs can capture more streptavidin, the SA-GMNPs with
either physical adsorption or covalent reaction were both stable in PBS buffer. In contrast, SA-GMNPs with covalent reaction was stable in SDS buffer, while most of the SA-GMNPs by physical adsorption would be eluted from the particles in SDS buffer. Therefore, the SA-GMNPs by covalent immobilization
were more suitable for fabrication of bead array. To evaluate the binding efficiency and capacity of DNA on SA-GMNPs, the capture of biotinylated oligonucleotide or PCR products on SA-GMNPs at different concentrations were examined. A magnetic beads array was fabricated by immobilizing DNA-MNPs
complexes onto a glass slide using a magnetic field. The synthesized DNA targets with different concentrations were detected with a detection limit of ∼0.05 nM, indicating the potential application of this MNPs array to high-throughput DNA detection.
Keywords: FUNCTIONALIZATION; GOLD MAGNETIC NANOPARTICLES; STREPTAVIDIN; SYNTHESIS
Document Type: Research Article
Publication date: 01 August 2010
- Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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