Live In-Vivo Neuroimaging Reveals the Transport of Lipophilic Cargo Through the Blood-Retina Barrier with Modified Amphiphilic Poly-N-Vinylpyrrolidone Nanoparticles
The blood-retina barrier (BRB), analogous to the blood-brain barrier, is a major hurdle for the passage of drugs from the blood to the central nervous system. Here, we designed polymeric nanoparticles from amphiphilic poly-/V-vinylpyrrolidone (Amph-PVP NPs) as a new carrier-system and
investigated their ability to pass the BRB using a live In-Vivo neuroimaging system for the retina in rats and ex-vivo wholemounted retinae preparation. Amph-PVP NPs were loaded with hydrophobic fluorescent markers as a surrogate for hydrophobic drugs. Linking these NPs with
the hydrophobic fluorescence marker Carboxyfluorescein-succinimidyl-ester (CFSE) to the surface, induced the passage of the cargo into the retina tissue. In particular, we observed a substantial internalization of the CFSE-linked NPs into blood cells. We propose surface- modified Amph-PVP
NPs as a potential new nano-carrier platform to target posterior eye and potentially brain diseases while camouflaged by blood cells.
Keywords: Amphiphilic Poly-N-Vinylpyrrolidone; Arivis Vision 4D; Blood-Retina Barrier; ICON; In-Vivo Neuroimaging; Nanoparticles; Retinal Diseases
Document Type: Research Article
Affiliations: 1: Institute of Medical Psychology, Otto von Guericke University of Magdeburg, Leipzigerstr. 44, 39120, Magdeburg, Germany 2: ArivisAG, Am Kabutzenhof 21, 18057, Rostock, Germany 3: Department of Biomaterials, Dmitry Mendeleev University of Chemical Technology of Russia, Miusskaya sq. 9, 125047, Moscow, Russia
Publication date: 01 May 2021
- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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