LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre

Kaszuba-Zwoińska Jolanta; Chorobik Paulina; Nowak Bernadeta; Ziomber Agata; Juszczak Kajetan; Zaraska Wiesław; Thor Piotr

doi:doi:10.11648/j.abb.20140203.12

LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, Zaraska Wiesław, Thor Piotr

Published in Advances in Bioscience and Bioengineering (Volume 2, Issue 3)

Received: 27 August 2014 Accepted: 11 September 2014 Published: 30 September 2014

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.

Published in	Advances in Bioscience and Bioengineering (Volume 2, Issue 3)
DOI	10.11648/j.abb.20140203.12
Page(s)	30-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Urothelial Cell Line, Monocytic Cell Line, Coculture, Apoptosis, Necrosis, Flow Cytometry, ICAM-1, IL-8,Pulsed Electromagnetic Field, VEGF-A

References

[1]	Birder L, Apodaca G, Groat W, Kanai A. Adrenergic- and capsaicin-evoked nitric oxide release from urothelium and afferent nerves in urinary bladder. American Journal of Physiology 275: F226–F229, 1998.
[2]	Butcher E. Leukocyte-Endothelial cell recognition: three (or more) steps to specificity and diversity. Cell 67: 1033-1036, 1991.
[3]	Carlos T, Harlan J. Leukocyte-Endothelial adhesion molecules. Blood 84: 2068-2101, 1994.
[4]	Delle Monache S, Angelucci A, Sanità P, Iorio R, Bennato F, Mancini F, Gualtieri G, Colonna RC. Inhibition of angiogenesis mediated by extremely low-frequency magnetic fields (ELF-MFs). PLoS One 8(11): e79309, 2013.
[5]	Dupont M, Spitsbergen J, Kim K, Tuttle J, Steers. Histological and neurotrophic changes triggered by varying models of bladder inflammation. Journal of Urology 166: 1111–1118, 2001.
[6]	Ferguson D, Kennedy I, Burton T. ATP is released from rabbit urinary bladder epithelial cells by hydrostatic pressure changes – a possible sensory mechanism? Journal of Physiology 505: 503–511, 1997.
[7]	Haraoka M, Hang L, Frendeus B, Godaly G, Burdick M, Strieter R, Svanborg C. Neutrophil recruitment and resistance to urinary tract infection. J Infect Dis 180: 1220–1229, 1999.
[8]	Hooton TM, Stamm WE. Diagnosis and treatment of uncomplicated urinary tract infection. Infect Dis Clin North Am 11: 551–581, 1997.
[9]	Hopper RA, VerHalen JP, Tepper O, Mehrara BJ, Detch R, Chang EI, Baharestani S, Simon BJ, Gurtner GC. Osteoblasts stimulated with pulsed electromagnetic fields increase HUVEC proliferation via a VEGF-A independent mechanism. Bioelectromagnetics 30(3): 189-97, 2009.
[10]	Horsley H, Malone-Lee J, Holland D, Tuz M, Hibbert A, Kelsey M, Kupelian A, Rohn JL. Enterococcus faecalis subverts and invades the host urothelium in patients with chronic urinary tract infection. PLoS One 8(12): e83637, 2013.
[11]	Juszczak K, Kaszuba-Zwoinska J, Thor PJ. Pulsating electromagnetic field stimulation of urothelial cells induces apoptosis and diminishes necrosis: new insight to magnetic therapy in urology. J Physiol Pharmacol 63(4): 397-401, 2012.
[12]	Kanasaki K, Yu W, von Bodungen M, Larigakis JD, Kanasaki M, Ayala de la Pena F, Kalluri R, Hill WG. Loss of β1-integrin from urothelium results in overactive bladder and incontinence in mice: a mechanosensory rather than structural phenotype. FASEB J 27(5): 1950-61, 2013.
[13]	Kaszuba-Zwoinska J, Chorobik P, Juszczak K, Zaraska W, Thor PJ. Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture. J Physiol Pharmacol 63(5): 537-45, 2012.
[14]	Kaszuba-Zwoińska J, Ciećko-Michalska I, Madroszkiewicz D, Mach T, Słodowska-Hajduk Z, Rokita E, Zaraska W, Thor P. Magnetic field anti-inflammatory effects in Crohn's disease depends upon viability and cytokine profile of the immune competent cells. J Physiol Pharmacol 59(1): 177-87, 2008.
[15]	Kaszuba-Zwoińska J, Zdziłowska E, Chorobik P, Słodowska-Hajduk Z, Juszczak K,Zaraska W, Thor P. Pulsing Electromagnetic Field and Death of Proliferating Peripheral Blood Mononuclear Cells from Patients with Acute Myelogenic Leukemia. Adv Clin Exp Med 20(6): 721–727, 2011.
[16]	Kaszuba-Zwoinska J, Wojcik K, Bereta M, Ziomber A, Pierzchalski P, Rokita E, Marcinkiewicz J, Zaraska W, Thor P. Pulsating electromagnetic field stimulation prevents cell death of puromycin treated U937 cell line. J Physiol Pharmacol 61(2): 201-5, 2010.
[17]	Keresteci AG, Leers WD. Indwelling catheter infection. Can Med Assoc J 109: 711–713, 1973.
[18]	Kowalewska PM, Burrows LL, Fox-Robichaud AE. Intravital microscopy of the murine urinary bladder microcirculation. Microcirculation 18(8): 613-22, 2011.
[19]	Lambert PA. Mechanisms of antibioticresistance in Pseudomonas aeruginosa. J R Soc Med 95(Suppl. 41): 22–26, 2002.
[20]	Niemelä J, Henttinen T, Yegutkin GG, Airas L, Kujari AM, Rajala P, Jalkanen S. IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5'-nucleotidase) up-regulation. J Immunol 172(3): 1646-53, 2004.
[21]	Ochodnicky P, Cruz C, Yoshimura N, Michel MC. Nerve growth factor in bladder dysfunction: contributing factor, biomarker, and therapeutic target . Neurourology and Urodynamics 30: 1227–1241, 2011.
[22]	Ochodnický P, Michel MB, Butter JJ, Seth J, Panicker JN, Michel MC. Bradykinin modulates spontaneous nerve growth factor production and stretch-induced ATP release in human urothelium. Pharmacol Res 70(1): 147-54, 2013.
[23]	Shahzad A, Knapp M, Lang I, Köhler G. Interleukin 8 (IL-8) - a universal biomarker? Int Arch Med 15: 3-11, 2010.
[24]	Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76: 301-14, 1994
[25]	Tielen P, Narten M, Rosin N, Biegler I, Haddad I, Hogardt M, Neubauer R, Schobert M, Wiehlmann L, Jahn D. Genotypic and phenotypic characterization of Pseudomonas aeruginosa isolates from urinary tract infections. Int J Med Microbiol 301: 282–292, 2011.
[26]	Vlaskovska M, Kasakov L, Rong W, Bodin P, Bardini M, Cockayne DA, Ford AP, Burnstock G. P2X3 knock-out mice reveal a major sensory role for urothelially released ATP Journal of Neuroscience 21: 5670–5677, 2001.
[27]	Yoshida M1, Inadome A, Maeda Y, Satoji Y, Masunaga K, Sugiyama Y, Murakami S. Non-neuronal cholinergic system in human bladder urothelium. Urology 67: 425–430, 2006.
[28]	Zarogoulidis P, Katsikogianni F, Tsiouda T, Sakkas A, Katsikogiannis N, Zarogoulidis K. Interleukin-8 and interleukin-17 for cancer. Cancer Invest 32(5): 197-205, 2014.

Cite This Article

Plain Text BibTeX RIS

APA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. (2014). LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Advances in Bioscience and Bioengineering, 2(3), 30-36. https://doi.org/10.11648/j.abb.20140203.12

Copy | Download

ACS Style

Kaszuba-Zwoińska Jolanta; Chorobik Paulina; Nowak Bernadeta; Ziomber Agata; Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv. BioSci. Bioeng. 2014, 2(3), 30-36. doi: 10.11648/j.abb.20140203.12

Copy | Download

AMA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv BioSci Bioeng. 2014;2(3):30-36. doi: 10.11648/j.abb.20140203.12

Copy | Download

@article{10.11648/j.abb.20140203.12,
  author = {Kaszuba-Zwoińska Jolanta and Chorobik Paulina and Nowak Bernadeta and Ziomber Agata and Juszczak Kajetan and Zaraska Wiesław and Thor Piotr},
  title = {LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {2},
  number = {3},
  pages = {30-36},
  doi = {10.11648/j.abb.20140203.12},
  url = {https://doi.org/10.11648/j.abb.20140203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20140203.12},
  abstract = {Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre
AU  - Kaszuba-Zwoińska Jolanta
AU  - Chorobik Paulina
AU  - Nowak Bernadeta
AU  - Ziomber Agata
AU  - Juszczak Kajetan
AU  - Zaraska Wiesław
AU  - Thor Piotr
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.abb.20140203.12
DO  - 10.11648/j.abb.20140203.12
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 30
EP  - 36
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20140203.12
AB  - Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.
VL  - 2
IS  - 3
ER  -

Copy | Download

Author Information

Kaszuba-Zwoińska Jolanta

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Chorobik Paulina

Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Nowak Bernadeta

Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Ziomber Agata

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Juszczak Kajetan

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Zaraska Wiesław
Thor Piotr

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. (2014). LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Advances in Bioscience and Bioengineering, 2(3), 30-36. https://doi.org/10.11648/j.abb.20140203.12

Copy | Download

ACS Style

Kaszuba-Zwoińska Jolanta; Chorobik Paulina; Nowak Bernadeta; Ziomber Agata; Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv. BioSci. Bioeng. 2014, 2(3), 30-36. doi: 10.11648/j.abb.20140203.12

Copy | Download

AMA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv BioSci Bioeng. 2014;2(3):30-36. doi: 10.11648/j.abb.20140203.12

Copy | Download

@article{10.11648/j.abb.20140203.12,
  author = {Kaszuba-Zwoińska Jolanta and Chorobik Paulina and Nowak Bernadeta and Ziomber Agata and Juszczak Kajetan and Zaraska Wiesław and Thor Piotr},
  title = {LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {2},
  number = {3},
  pages = {30-36},
  doi = {10.11648/j.abb.20140203.12},
  url = {https://doi.org/10.11648/j.abb.20140203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20140203.12},
  abstract = {Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre
AU  - Kaszuba-Zwoińska Jolanta
AU  - Chorobik Paulina
AU  - Nowak Bernadeta
AU  - Ziomber Agata
AU  - Juszczak Kajetan
AU  - Zaraska Wiesław
AU  - Thor Piotr
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.abb.20140203.12
DO  - 10.11648/j.abb.20140203.12
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 30
EP  - 36
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20140203.12
AB  - Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.
VL  - 2
IS  - 3
ER  -

Copy | Download