help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on May 13, 2005, doi:10.1164/rccm.200412-1621OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Supplement
Right arrow All Versions of this Article:
200412-1621OCv1
172/4/433    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grissell, T. V.
Right arrow Articles by Gibson, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grissell, T. V.
Right arrow Articles by Gibson, P. G.
American Journal of Respiratory and Critical Care Medicine Vol 172. pp. 433-439, (2005)
© 2005 American Thoracic Society
doi: 10.1164/rccm.200412-1621OC

Original Article

Interleukin-10 Gene Expression in Acute Virus-induced Asthma

Terry V. Grissell, Heather Powell, Darren R. Shafren, Michael J. Boyle, Michael J. Hensley, Peter D. Jones, Bruce F. Whitehead and Peter G. Gibson

School of Medical Practice and Population Health, University of Newcastle, Callaghan; Department of Respiratory and Sleep Medicine, Hunter Medical Research Institute, and Immunology and Infectious Diseases Unit, John Hunter Hospital, New Lambton; Picornaviral Research Unit, Royal Newcastle Hospital, Newcastle; and John Hunter Children's Hospital, Kaleidoscope, New Lambton, New South Wales, Australia

Correspondence and requests for reprints should be addressed to Peter G. Gibson, M.B.B.S., Level 3 HMRI, John Hunter Hospital, Locked Bag 1, Hunter Region Mail Centre, New Lambton, NSW 2310, Australia. E-mail: peter.gibson{at}hnehealth.nsw.gov.au

Rationale: Virus-induced asthma is characterized by marked neutrophil influx and eosinophil degranulation, suggesting a mode of immunopathogenesis different from that of allergen-induced asthma. Objectives: This study compared induced sputum cytokine responses in subjects with severe asthma exacerbation and respiratory virus infection with those of patients with stable asthma, healthy control subjects, and virus-infected nonasthmatic subjects. Methods: Subject infection status and pulmonary history were established on the basis of common cold and asthma questionnaires, and lung function and atopy tests were performed. Respiratory virus infection was diagnosed by cell culture and direct polymerase chain reaction, using induced sputum. The induced sputum cellular profile was examined and cytokine gene expression was assessed by quantitative real-time polymerase chain reaction. Results: A respiratory virus was detected in 78% of subjects with acute asthma. Specific viruses detected were rhinovirus (83%), influenza (15%), enterovirus (4%), and respiratory syncytial virus (2%). Virus-infected subjects with acute asthma or no asthma had increased RANTES (regulated on activation, normal T cell expressed and secreted) and macrophage inflammatory protein-1{alpha} messenger RNAs compared with other groups. Interleukin (IL)-10 mRNA was significantly increased in virus-infected acute asthma and reduced on recovery from acute asthma. IL-5, eotaxin, and IL-8 mRNA transcripts were similar across groups. Conclusions: Asthma exacerbation triggered by respiratory virus infection is characterized by increased IL-10 gene expression that may explain the suppressed eosinophil influx in acute asthma. Airway neutrophilia due to respiratory virus infection is associated with chemokine gene expression involving RANTES and macrophage inflammatory protein-1{alpha}.

Key Words: asthma • cytokines • gene expression • viruses




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Papi, M. Contoli, P. Gasparini, L. Bristot, M. R. Edwards, M. Chicca, M. Leis, A. Ciaccia, G. Caramori, S. L. Johnston, et al.
Role of Xanthine Oxidase Activation and Reduced Glutathione Depletion in Rhinovirus Induction of Inflammation in Respiratory Epithelial Cells
J. Biol. Chem., October 17, 2008; 283(42): 28595 - 28606.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. D. Message, V. Laza-Stanca, P. Mallia, H. L. Parker, J. Zhu, T. Kebadze, M. Contoli, G. Sanderson, O. M. Kon, A. Papi, et al.
Rhinovirus-induced lower respiratory illness is increased in asthma and related to virus load and Th1/2 cytokine and IL-10 production
PNAS, September 9, 2008; 105(36): 13562 - 13567.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
B G G Oliver, S Lim, P Wark, V Laza-Stanca, N King, J L Black, J K Burgess, M Roth, and S L Johnston
Rhinovirus exposure impairs immune responses to bacterial products in human alveolar macrophages
Thorax, June 1, 2008; 63(6): 519 - 525.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. L. Simpson, H. Powell, M. J. Boyle, R. J. Scott, and P. G. Gibson
Clarithromycin Targets Neutrophilic Airway Inflammation in Refractory Asthma
Am. J. Respir. Crit. Care Med., January 15, 2008; 177(2): 148 - 155.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
P. G Gibson
What do non-eosinophilic asthma and airway remodelling tell us about persistent asthma?
Thorax, December 1, 2007; 62(12): 1034 - 1036.
[Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
S. L. Johnston
Innate Immunity in the Pathogenesis of Virus-induced Asthma Exacerbations
Proceedings of the ATS, July 1, 2007; 4(3): 267 - 270.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
L. G. Wood, H. Powell, T. Grissell, T. T.D. Nguyen, D. Shafren, M. Hensley, and P. G. Gibson
Persistent Airway Obstruction After Virus Infection Is Not Associated With Airway Inflammation
Chest, February 1, 2007; 131(2): 415 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. M. Singh, P. E. Moore, J. E. Gern, R. F. Lemanske Jr., and T. V. Hartert
Bronchiolitis to Asthma: A Review and Call for Studies of Gene-Virus Interactions in Asthma Causation
Am. J. Respir. Crit. Care Med., January 15, 2007; 175(2): 108 - 119.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
P. Mallia and S. L. Johnston
How viral infections cause exacerbation of airway diseases.
Chest, October 1, 2006; 130(4): 1203 - 1210.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
P A B Wark and P G Gibson
Asthma exacerbations {middle dot} 3: Pathogenesis.
Thorax, October 1, 2006; 61(10): 909 - 915.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
A M Singh and W W Busse
Asthma exacerbations {middle dot} 2: Aetiology
Thorax, September 1, 2006; 61(9): 809 - 816.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
H. K. Reddel and D. J. Barnes
Pharmacological strategies for self-management of asthma exacerbations.
Eur. Respir. J., July 1, 2006; 28(1): 182 - 199.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. E. Wenzel and R. Covar
Update in asthma 2005.
Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 698 - 706.
[Full Text] [PDF]

Home page
 ERRHome page
S. L. Johnston
Impact of viruses on airway diseases
Eur. Respir. Rev., December 1, 2005; 14(95): 57 - 61.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. T. Holgate
Exacerbations: The Asthma Paradox
Am. J. Respir. Crit. Care Med., October 15, 2005; 172(8): 941 - 943.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. W. Busse and J. E. Gern
Is Interleukin-10 a "10" in Virus-provoked Asthma?
Am. J. Respir. Crit. Care Med., August 15, 2005; 172(4): 405 - 406.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2005 American Thoracic Society