Special issue published for the:
14th International Chromosome Conference.
September 4-8, 2001, Würzburg, Germany.
Editors: Michael Schmid, Würzburg; Harold P. Klinger, New York, N.Y.

Original Article

The combination of SKY and specific loci detection with FISH or immunostaining
C.J. Ye^a, W. Lu^b, G. Liu^b, S.W. Bremer^b, Y.A. Wang^c,d, P. Moens^f, M. Hughes^b,c,d, S.A. Krawetz^b,e, H.H.Q. Heng<sup>b,c,d

a</sup>SeeDNA Biotech Inc, Windsor, Ontario (Canada);
^bCenter for Molecular Medicine and Genetics,
^cDepartment of Pathology,
^dKarmanos Cancer Institute,
^eDepartment of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit MI (USA);
^fBiology Department, York University, Toronto, Ontario (Canada)

Address of Corresponding Author

Cytogenet Cell Genet 2001;93:195-202 (DOI: 10.1159/000056984)

  Abstract.

Spectral karyotyping (SKY) represents an effective tool to detect individual chromosomes and analyze major karyotype abnormalities within an entire genome. We have tested the feasibility of combining SKY and FISH/protein detection in order to combine SKY's unique abilities with specific loci detection. Our experimental results demonstrate that various combined protocols involving SKY, FISH and immunostaining work well when proper procedures are used. This combined approach allows the tracking of key genes or targeted chromosome regions while monitoring changes throughout the whole genome. It is particularly useful when simultaneously monitoring the behavior of both protein complexes and DNA loci within the genome. The details of this methodology are described and systematically tested in this communication.   

Copyright © 2001 S. Karger AG, Basel

  Author Contacts

Request reprints from Henry H.Q. Heng, Center for Molecular Medicine andGenetics, Wayne State University School of Medicine,5107 Biological Science Building, 5047 Gullen Mall,Detroit, MI 48202 (USA); telephone: 313-577-9544; fax: 313-577-6200;e-mail: hheng@cmb.biosci.wayne.edu

  Article Information

Supported by the start-up fund for H.H.Q.H. from the Center for Molecular Medicine and Genetics, Wayne State School of Medicine, the virtual discovery fund for H.H.Q.H. and S.A.K. from Karmanos Cancer Institute, and the research and development fund for C.J.Y. from SeeDNA Biotech Inc., Windsor, Ontario.

Received: Received 29 January 2001;
accepted 22 May 2001.
Number of Print Pages : 8
Number of Figures : 6, Number of Tables : 1, Number of References : 17