Paper

Spectral karyotyping of mouse cell line WMP2
G. Liu^a, W. Lu^a, S. Bremer^a, H. Hameister^b, B. Schreiner^b, M. Hughes^a, H.H.Q. Heng<sup>a

a</sup>Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI (USA), and
^bDepartment of Human Genetics, University Ulm, Ulm (Germany)

Address of Corresponding Author

Cytogenet Cell Genet 2000;90:271-274 (DOI: 10.1159/000056787)

  Abstract.

We have evaluated the mouse cell line WMP2 using both GTG-banding analysis and spectral karyotyping to verify the reliability of using this established cell line derived from WMP/WMP mice. The WMP cell lines contain easily identifiable metacentric fusion chromosomes and are used extensively for gene mapping. Because of karyotypical changes in the WMP1 cell line, WMP2 was examined. Our results demonstrate that WMP2 is stable during culture, and the karyotype is simple and easy to use. Based on the findings discussed in this paper, we recommend the use of the WMP2 cell line for future prospective gene mapping in the mouse.   

Copyright © 2000 S. Karger AG, Basel

  Author Contacts

Request reprints from Dr. Henry H.Q. Heng,Center for Molecular Medicine and Genetics,Wayne State University School of Medicine,5107 Biological Science Building,5047 Gullen Mall, Detroit, MI 48202 (USA); telephone: 313-577-1125;fax: 313-577-6200; e-mail: hheng@cmb.biosci.wayne.edu.

  Article Information

Supported by a startup fund for H.H. from the Center for Molecular Medicine and Genetics, Wayne State University School of Medicine. Additional support was provided by the Genomics/Genetics core, Karmanos Cancer Institute.

Received: Received 2 June 2000;
revision accepted 24 July 2000.
Number of Print Pages : 4
Number of Figures : 2, Number of Tables : 1, Number of References : 10