Intestinal Mucosal Damage and Repair
5th Eisai Biwako Symposium
November 19-20, 1999, Lake Biwa, Shiga, Japan
Guest Editor: Tadao Bamba, Shiga

New Aspects and Mucosal Repair

Role of Complement Activation and Mast Cell Degranulation in the Pathogenesis of Rapid Intestinal Ischemia/Reperfusion Injury in Rats
Akira Andoh, Yoshihide Fujiyama, Yoshio Araki, Toshio Kimura, Tomoyuki Tsujikawa, Tadao Bamba

Department of Internal Medicine, Shiga University of Medical Science, Seta-Tsukinowa, Otsu, Japan

Address of Corresponding Author

Digestion 2001;63:103-107 (DOI: 10.1159/000051920)

  Key Words

• Anaphylatoxin
• Mucosal permeability
• Ws/Ws rat

  Abstract

The aim of this study is to define the putative role of complement activation and mucosal mast cell (MMC) degranulation in the pathogenesis of rapid ischemia-reperfusion (I/R) injury. We prepared complement activity-depleted rats by the administration of the anti-complementary agent K-76COONa. To assess the role of MMC degranulation, we used the MMC stabilizer MAR-99 and genetically mast cell-deficient Ws/Ws rats. Autoperfused segments of the jejunum were exposed to 60 min of ischemia, followed by 60 min reperfusion. The epithelial permeability was assessed by ⁵¹Cr-EDTA clearance rate, and the number of MMC was immunohistochemically assessed. I/R treatment induced a marked increase in mucosal permeability and MMC degranulation. The treatment with K-76COONa and MAR-99 significantly attenuated these changes. Furthermore, in Ws/Ws rats the increase in mucosal permeability and MMC degranulation was significantly attenuated. These findings indicate the role of complement activation and MMC activation in the pathogenesis of rapid intestinal I/R injury. A regulation of the complement activation and MMC degranulation may be one of the clinical strategies for prevention of I/R-induced mucosal injury.

Copyright © 2001 S. Karger AG, Basel

  Author Contacts

Akira Andoh, MD
Department of Internal Medicine
Shiga University of Medical Science
Seta-Tukinowa, Otsu 520-2192 (Japan)
Tel. +81 77 548 2217, Fax +81 77 548 2219, E-Mail andoh@belle.shiga-med.ac.jp

  Article Information

Number of Print Pages : 5
Number of Figures : 3, Number of Tables : 0, Number of References : 16