Inability to Clonally Expand Fetal Progenitors from Maternal Blood

Vol. 17, No. 2, 2002

Free Abstract Article (References) Article (PDF 172 KB)

Paper

Inability to Clonally Expand Fetal Progenitors from Maternal Blood
Bernhard Zimmermann, Wolfgang Holzgreve, Xiao Yan Zhong, Sinuhe Hahn

Department of Obstetrics and Gynaecology and Department of Research, University of Basel, Switzerland

Address of Corresponding Author

Fetal Diagn Ther 2002;17:97-100 (DOI: 10.1159/000048016)

Key Words

Fetal cells
Maternal blood culture
Polymerase chain reaction

Abstract

Objectives: To confirm the recent report of the culture of single clones of fetal progenitor cells from maternal blood. Methods: Hemopoietic progenitor cells were cultured from 16 blood samples obtained from women pregnant with a male singleton fetus. Single colonies were isolated by micro-manipulation and examined by multiplex real-time PCR. Results: Of a total of 1,674 colonies examined, 1,648 were identified as being maternal. No colonies were detected of fetal origin. Conclusions: The clonal expansion of single fetal colonies from the maternal circulation is not feasible by current methods.

Author Contacts

Sinuhe Hahn, PhD
Department of Obstetrics and Gynaecology and Department of Research
University of Basel, Schanzenstrasse 46
CH-4031 Basel (Switzerland)
Tel. +41 61 325 9224, Fax +41 61 325 9399, E-Mail shahn@uhbs.ch

Article Information

This work was supported in part by Swiss National Science Foundation Grant number 3200-055614.98/1 and NIH (USA) Contract number N01-HD-4-3202.

Received: Received: August 30, 2001
Accepted: August 30, 2001
Number of Print Pages : 4
Number of Figures : 1, Number of Tables : 1, Number of References : 17

Medline Abstract (ID 11844913)

Download Citation

For non-native English speakers and international authors who would like assistance with their writing before submission, we suggest American Journal Experts for their research paper editing service.