Original Paper

Effects of Human Alpha-1-Acid Glycoprotein on Aminonucleoside- Induced Minimal Change Nephrosis in Rats
Eva-Maria Muchitsch^a, Ludwig Pichler^a, Hans Peter Schwarz^a, Walter Ulrich<sup>b

a</sup>Research Laboratories, Immuno AG, und
^bJakob-Erdheim-Institut für Pathologie und Klinische Bakteriologie, Krankenhaus der Stadt Wien-Lainz, Wien, Österreich

Address of Corresponding Author

Nephron 1999;81:194-199 (DOI: 10.1159/000045276)

  Key Words

• Alpha-1-acid glycoprotein
• Puromycin aminonucleoside
• Minimal change nephrosis
• Rat

  Abstract

A minimal change nephrosis was induced in rats by a single intraperitoneal injection of puromycin aminonucleoside (100 mg/kg). This resulted in increased urine protein output, plasma creatinine, blood urine nitrogen, and relative kidney weight. Electronoptically, there was a retraction of the glomerular podocytic foot processes. When human ₁-acid glycoprotein was injected at 600 mg/kg intravenously on experimental days 6, 7, 8, and 9 into these animals, urine protein output decreased significantly, and the number of podocytic foot processes increased significantly. ₁-Acid glycoprotein is rich in sialic acid and largely negatively charged. Its therapeutic role in nephrosis, which is characterized by a loss of sialic acid and a loss of negative charge, thereby leading to a loss of permselectivity, is discussed.

  Author Contacts

L. Pichler
Immuno AG, Industriestrasse 67
A-1221 Vienna (Austria)
Tel. +43 1 20100 2090, Fax +43 1 20100 525
E-Mail mikyses@baxter.com

  Article Information

Accepted: August 24, 1998
Number of Print Pages : 6
Number of Figures : 5, Number of Tables : 2, Number of References : 14