Original Paper

Expression of VCAM-1 in Lymphocytes during the Process of Apoptosis
N. Ishiyama^a, M. Kitagawa^a, H. Takahashi^b, T. Kina^c, K. Hirokawa<sup>a

a</sup> Department of Pathology and Immunology, Faculty of Medicine, Tokyo Medical and Dental University, Tokyo,
^b Mitsubishi Kasei Institute of Life Sciences, Tokyo, and
^c Department of Immunology, Chest Disease Research Institute, Kyoto University, Japan

Address of Corresponding Author

Pathobiology 1998;66:274-283 (DOI: 10.1159/000028034)

  Key Words

• Apoptosis
• VCAM-1
• Thymocytes
• Spleen cells
• Lymphoid cell lines

  Abstract

The administration of corticosteroids induced apoptosis of thymocytes in vivo. Among various adhesion molecules examined, vascular cell adhesion molecule-1 (VCAM-1, CD106) was shown to be strongly expressed in these apoptotic cells. Flow cytometric analysis also showed the expression of VCAM-1 in apoptotic thymocytes. An RT-PCR study demonstrated the expression of VCAM-1 mRNA in thymocytes. Splenic lymphocytes and other lymphoid cell lines also expressed VCAM-1 during the process of apoptosis. VCAM-1 mRNA expression was also observed in RT-PCR performed on these cell lines.

  Author Contacts

Dr. N. Ishiyama
Department of Pathology and Immunology, Faculty of Medicine
Tokyo Medical and Dental University
5-45, Yushima, 1-chome, Bunkyo-ku, Tokyo 113-8519 (Japan)
Tel. +81 3 5803 5176, Fax +81 3 5803 0123, E-Mail ishipth2@med.tmd.ac.jp

  Article Information

Received: Received: April 2, 1998
Accepted: May 1, 1998
Number of Print Pages : 10
Number of Figures : 9, Number of Tables : 0, Number of References : 34