Paper

Identification of the mouse `-COP golgi component as a spermatocyte autoantigen in scleroderma and mapping of its gene Copb2 to mouse Chromosome 9
M. Tarsounas^a, H.H.Q. Heng^b, C.J. Ye^c, R.E. Pearlman^a, P.B. Moens<sup>a

a</sup>Department of Biology, York University, Toronto ON (Canada);
^bCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit MI (USA);
^cSeeDNA Biotech Inc., Windsor, ON (Canada)

Address of Corresponding Author

Cytogenet Cell Genet 1999;87:201-204 (DOI: 10.1159/000015467)

  Abstract.

In an immunological screening of a mouse testicular cDNA library with a human CREST serum we isolated five overlapping cDNA clones encoding the mouse homolog of a Golgi coatomer complex protein (accession number AF043120), designated '-COP in bovine and p102 in humans. We generated antibodies against this protein which specifically recognize the Golgi apparatus of mouse spermatocytes. FISH analyses assigned the '-COP gene Copb2 to mouse Chromosome 9, region E3-F1. Our results demonstrate that CREST sera can contain antibody components against Golgi proteins as well as against nuclear proteins.    

Copyright © 2000 S. Karger AG, Basel

  Author Contacts

Request reprints from Peter B. Moens, Department of Biology, York University,4700 Keele Street, Toronto, ON, M3J 1P3 (Canada); telephone: (416) 736-5358;fax: (416) 736-5731; e-mail: moens@yorku.ca
Present address of M.T.: Wellcome-CRC Institute, Cambridge (UK).

  Article Information

Supported by grants from NSERC to R.E.P. and P.B.M.

Received: Received 29 July 1999;
manuscript accepted 18 August 1999.
Number of Print Pages : 4
Number of Figures : 3, Number of Tables : 0, Number of References : 13