Paper

Mapping and complex expression pattern of the human NPAP60L nucleoporin gene
V. Trichet^a, D. Shkolny^a, I. Dunham^b, D. Beare^b, H.E. McDermid<sup>a

a</sup>Department of Biological Sciences, University of Alberta, Edmonton (Canada), and
^bThe Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge (UK)

Address of Corresponding Author

Cytogenet Cell Genet 1999;85:221-226 (DOI: 10.1159/000015297)

  Abstract.

From a clone mapping to human chromosome 22q13.3, we have identified NPAP60L, the human homolog of the rat nuclear pore-associated protein gene, Npap60. The expression pattern of the human copy is much more complex that that of the rat, although conservation of the potential specific function of NPAP60L in male germ cells can be seen for one of the five transcripts. The exon-intron organization of the NPAP60L gene shows the presence of at least three alternate 3' ends, and Northern analysis indicates the possible presence of alternate 5' ends. Somatic cell hybrid mapping revealed additional related copies of NPAP60L on human chromosomes 5, 6, and 14, although it is not known if these are functional genes.

  Author Contacts

Request reprints from Dr. Heather E. McDermid, Department of Biological Sciences, University of Alberta, Edmonton (Canada) T6G 2E9; telephone: 780-492-5377; fax: 780-492-9234; e-mail: hmcdermi@gpu.srv.ualberta.ca.

  Article Information

Supported by an operating grant from the Canadian Genome Analysis and Technology Program/Medical Research Council (to H.E.M.). The work at The Sanger Center is funded by the Wellcome Trust. V.T.'s present address is CNRS UPR1142, Institut de Biologie, 4 bd Henri IV, 34060 Montpellier (France). The sequences described in this paper have been submitted to GenBank/EMBL under the accession number AF107840.

Received: Received 5 January 1999;
revision accepted 23 March 1999.
Number of Print Pages : 6
Number of Figures : 3, Number of Tables : 0, Number of References : 19