This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kim, J. Articles by Ho, S.-M. Search for Related Content PubMed PubMed Citation Articles by Kim, J. Articles by Ho, S.-M. Related Collections Clinical Intervention Clinical Intervention: Biomarkers

Changes in Serum Proteomic Patterns by Presurgical -Tocopherol and L-Selenomethionine Supplementation in Prostate Cancer

Departments of ¹ Genitourinary Medical Oncology, ² Pathology, ³ Clinical Cancer Prevention, ⁴ Urology, and ⁵ Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston, Texas; ⁶ Department of Surgery, Division of Urology, University of Massachusetts Medical School, Worcester, Massachusetts; and ⁷ Prostate and Urologic Cancer Research Group, Division of Cancer Prevention, National Cancer Institute, Bethesda, Maryland

Requests for reprints: Shuk-Mei Ho, University of Massachusetts Medical School, Lazare Research Building, Room 504, 364 Plantation Street, Worcester, MA 01605-2324. Phone: 508-856-1909; Fax: 508-856-8699. E-mail: shuk-mei.ho{at}umassmed.edu

Background: Evidence of the chemopreventive effects of the dietary antioxidants -tocopherol (vitamin E) and L-selenomethionine (selenium) comes from secondary analysis of two phase III clinical trials that found treatment with these antioxidants reduced the incidence of prostate cancer. To determine the effects of selenium and vitamin E in blood and prostate tissue, we undertook a preoperative feasibility study complementary to the currently ongoing Selenium and Vitamin E Cancer Prevention Trial.

Methods: Forty-eight patients with clinically localized prostate cancer enrolled on this 2 x 2 factorial design study were randomized to take selenium, vitamin E, both, or placebo for 3 to 6 weeks before prostatectomy. Sera were collected from patients before and after dietary supplementation. Thirty-nine patients were evaluable, and 29 age-matched disease-free men served as controls. Mass profiling of lipophilic serum proteins of lower molecular weight (2-13.5 kDa) was conducted, and mass spectra data were analyzed using custom-designed software.

Results: Weighted voting analyses showed a change in sera classification from cancerous to healthy for some patients with prostate cancer after dietary intervention. ANOVA analysis showed significantly different treatment effects on prediction strength changes among the four groups at a 95% confidence level. Eliminating an outlying value and performing post hoc analysis using Fisher's least significant difference method showed that effects in the group treated with the combination were significantly different from those of the other groups.

Conclusion: In sera from patients with prostate cancer, selenium and vitamin E combined induced statistically significant proteomic pattern changes associated with prostate cancer–free status.

This article has been cited by other articles:

				W. Demark-Wahnefried, S. L George, B. R Switzer, D. C Snyder, J. F Madden, T. J Polascik, M. T Ruffin IV, and R. T Vollmer Overcoming challenges in designing and implementing a phase II randomized controlled trial using a presurgical model to test a dietary intervention in prostate cancer Clinical Trials, June 1, 2008; 5(3): 262 - 272. [Abstract] [PDF]

				G. J. Kelloff, S. M. Lippman, A. J. Dannenberg, C. C. Sigman, H. L. Pearce, B. J. Reid, E. Szabo, V. C. Jordan, M. R. Spitz, G. B. Mills, et al. Progress in Chemoprevention Drug Development: The Promise of Molecular Biomarkers for Prevention of Intraepithelial Neoplasia and Cancer--A Plan to Move Forward Clin. Cancer Res., June 15, 2006; 12(12): 3661 - 3697. [Abstract] [Full Text] [PDF]