can-23-0883_supplementary_information_suppsi.docx (119.39 kB)
Supplementary Information from Prostate Cancer Progression Relies on the Mitotic Kinase Citron Kinase
journal contribution
posted on 2023-12-15, 08:40 authored by Chitra Rawat, Salma Ben-Salem, Nidhi Singh, Gaurav Chauhan, Anja Rabljenovic, Vishwa Vaghela, Varadha Balaji Venkadakrishnan, Jonathan D. Macdonald, Ujjwal R. Dahiya, Yara Ghanem, Salam Bachour, Yixue Su, Adam D. DePriest, Sanghee Lee, Michelle Muldong, Hyun-Tae Kim, Sangeeta Kumari, Malyn May Valenzuela, Dingxiao Zhang, Qiang Hu, Eduardo Cortes Gomez, Scott M. Dehm, Amina Zoubeidi, Christina A.M. Jamieson, Marlo Nicolas, Jesse McKenney, Belinda Willard, Eric A. Klein, Cristina Magi-Galluzzi, Shaun R. Stauffer, Song Liu, Hannelore V. HeemersSupplementary Information file
Funding
National Institutes of Health (NIH)
Case Comprehensive Cancer Center, Case Western Reserve University (Case CCC)
Dr. Ralph and Marian Falk Medical Research Trust (Falk Medical Research Trust)
VeloSano
History
ARTICLE ABSTRACT
Prostate cancer remains the second leading cause of cancer death in men in Western cultures. A deeper understanding of the mechanisms by which prostate cancer cells divide to support tumor growth could help devise strategies to overcome treatment resistance and improve survival. Here, we identified that the mitotic AGC family protein kinase citron kinase (CIT) is a pivotal regulator of prostate cancer growth that mediates prostate cancer cell interphase progression. Increased CIT expression correlated with prostate cancer growth induction and aggressive prostate cancer progression, and CIT was overexpressed in prostate cancer compared with benign prostate tissue. CIT overexpression was controlled by an E2F2–Skp2–p27 signaling axis and conferred resistance to androgen-targeted treatment strategies. The effects of CIT relied entirely on its kinase activity. Conversely, CIT silencing inhibited the growth of cell lines and xenografts representing different stages of prostate cancer progression and treatment resistance but did not affect benign epithelial prostate cells or nonprostatic normal cells, indicating a potential therapeutic window for CIT inhibition. CIT kinase activity was identified as druggable and was potently inhibited by the multikinase inhibitor OTS-167, which decreased the proliferation of treatment-resistant prostate cancer cells and patient-derived organoids. Isolation of the in vivo CIT substrates identified proteins involved in diverse cellular functions ranging from proliferation to alternative splicing events that are enriched in treatment-resistant prostate cancer. These findings provide insights into the regulation of aggressive prostate cancer cell behavior by CIT and identify CIT as a functionally diverse and druggable driver of prostate cancer progression. The poorly characterized protein kinase citron kinase is a therapeutic target in prostate cancer that drives tumor growth by regulating diverse substrates, which control several hallmarks of aggressive prostate cancer progression.See related commentary by Mishra et al., p. 4008Usage metrics
Categories
Keywords
Cell CycleSignal transduction pathwaysDrug ResistanceNovel mechanismsDrug TargetsProtein kinase & phosphatase drug targetsGene RegulationPosttranscriptional and translational controlGenitourinary CancersProstate cancerOncogenes & Tumor SuppressorsOncogenesProgression, Invasion & MetastasisTumor progressionTranslational Research
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC