Journal of Biomedicine and Biotechnology 
Volume 2 (2002), Issue 3, Pages 151-157
doi:10.1155/S1110724302204039
Research article

Quantitative Trait Loci Associated with Foliar Trigonelline Accumulation in Glycine Max L

Youngkoo Cho,1,2,4 Victor N. Njiti,3,4 Xinbo Chen,1,4,5 Kanokporn Triwatayakorn,3,4 My Abdelmajid Kassem,3,4 Khalid Meksem,3,4 David A. Lightfoot,3,4 and Andrew J. Wood1,4

 1Department of Plant Biology, Southern Illinois University-Carbondale, Carbondale 62901, IL, USA
2Department of Biology, Eastern New Mexico University, Portales 88130, NM, USA
3Department of Plant, Soil, and General Agriculture, Southern Illinois University-Carbondale, Carbondale 62901, IL, USA
4Center for Excellence in Soybean Research, Teaching and Outreach, Southern Illinois University-Carbondale, Carbondale 62901, IL, USA
5Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette 47907, IN, USA
Received 27 February 2002; Revised 15 April 2002; Accepted 16 April 2002

Abstract

The objective of this study was to utilize a Glycine max RIL population to (1) evaluate foliar trigonelline (TRG) content in field-grown soybean, (2) determine the heritability of TRG accumulation, and (3) identify DNA markers linked to quantitative trait loci (QTLs) conditioning variation in TRG accumulation. Frequency distributions of 70 recombinant inbred lines showed statistically no significant departure from normality (P>.05) for TRG accumulation measured at pod development stage (R4). Six different molecular linkage groups (LGs) (B2, C2, D2, G, J, and K) were identified to be linked to QTLs for foliar TRG accumulation. Two unique microsatellite markers (SSR) on two different linkage groups identified QTL significantly associated with foliar TRG accumulation: a region on LG J (Satt285) (P=.0019, R2=15.9%) and a second region on LG C2 (Satt079) (P=.0029, R2=13.4%).