Development of Biodegradable Delivery Systems Containing Novel 1,2,4-Trioxolane Based on Bacterial Polyhydroxyalkanoates

Lipaikin, Sergei Y.; Yaremenko, Ivan A.; Terent’ev, Alexander O.; Volova, Tatiana G.; Shishatskaya, Ekaterina I.

doi:https://doi.org/10.1155/2022/6353909

Advances in Polymer Technology

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Abstract Introduction Methods Results and Discussion Conclusion Data Availability Conflicts of Interest Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2022 | Article ID 6353909 | https://doi.org/10.1155/2022/6353909

Development of Biodegradable Delivery Systems Containing Novel 1,2,4-Trioxolane Based on Bacterial Polyhydroxyalkanoates

Sergei Y. Lipaikin,¹Ivan A. Yaremenko,²Alexander O. Terent’ev,²Tatiana G. Volova,^1,3and Ekaterina I. Shishatskaya¹

Academic Editor: Chiara Gualandi

Received14 Dec 2021

Revised07 Feb 2022

Accepted09 Feb 2022

Published28 Feb 2022

Abstract

In this work, delivery systems in the form of microparticles and films containing 1,2,4-trioxolane (ozonide, OZ) based on polyhydroxyalkanoates (PHAs) were developed. Main systems’ characteristics were investigated: the particle yield, average diameter, zeta potential, surface morphology, loading capacity, and drug release profile of microparticles, as well as surface morphology and release profiles of OZ-containing films. PHA-based OZ-loaded microparticles have been found to have satisfactory size, zeta potential, and ozonide loading-release behavior. It was noted that OZ content influenced the surface morphology of obtained systems.

1. Introduction

Over the past decade, cyclic organic peroxides have been intensively researched by chemists and pharmacologists. The discovery of the natural peroxide artemisinin [1–3], which has antimalarial properties, was the beginning of the development of biologically active peroxides (Figure 1). It has been found that cyclic peroxides have antimalarial [4–12], antiparasitic [13–19], anticancer [20–31], antiviral [32–34], and fungicidal [18, 35, 36] activities. On the basis of the fully synthetic peroxide arterolane, which contains a 1,2,4-trioxolane (ozonide) fragment, has been created an antimalarial drug of the latest generation—Synriam [37]. It was recently found that artesunate is able to inhibit NL63 α-coronavirus and OC43 β-coronavirus, while ozonides OZ277 and OZ418 are able to inhibit both α-coronavirus NL63, β-coronavirus OC43, and SARS-CoV-2 [38].

Controlled release drug delivery systems based on biodegradable polymeric films and microparticles can reduce detrimental side effects of biologically active substances. The matrix of such systems must be biocompatible, in the case of medical use, and environmentally safe and friendly, for use in agriculture.

Poly(lactic-co-glycolic acid) (PLGA) [39–41], polycaprolactone (PCL) [42, 43], and polyhydroxyalkanoates (PHAs) [44, 45] are widely used as a polymer matrix for depot systems. One of the most perspective matrices of such systems is PHAs, bacterial polyesters that possess biodegradability in biological media, biocompatibility, and nontoxicity. PHAs are water-insoluble semicrystalline substances suitable for medical, agricultural, and industrial applications [46–48].

Poly-3-hydroxybutyrate (P3HB) is the first well-described and studied microbial short-chain length polyhydroxyalkanoate (Figure 2(a)). The P3HB degree of crystallinity is about 72%, the melting point is between 174 and 179°C, and it also has a wide range of molecular weight distribution, depending on the cultivation procedure [49]. P3HB copolymers with poly-3-hydroxyvalerate, P3HV (PHBV, the molar fraction of P3HV varies from 10% to 80%), are recognized as more elastic and plastic material, surpassing P3HB in absolute strength and Young’s modulus. These properties make PHBV more suitable in bioengineering, for obtaining particles, films, scaffolds, and surgical suture (maintaining high biocompatibility for different biological systems and complete biodegradation) [47]. Anti-inflammatory [50], antibacterial [51], antitumor [52], herbicidal [53], and other drugs have been successfully encapsulated in the PHA matrix.

(a)

(b)

The natural antimalarial drug artemisinin (ART) is the most commonly used substance in the development of peroxide-containing controlled release systems. Polymer-based systems for ART delivery are usually based on PLGA [54], PCL [55], and cyclodextrin [56].

As far as we know, there are no data in the literature about the encapsulation of synthetic peroxides into the PHA matrix. In this work, we have chosen the synthetic ozonide (OZ) as the model object. OZ (Figure 2(b)) is a hydrophobic white powder that dissolves in DMSO, chloroform, and acetonitrile. The absorption maxima of the substance are in the UV region (268 and 276.5 nm). Since ozonides related to OZ exhibit anticancer [25–27], antiparasitic [57], and fungicidal activity [36], PHA-microparticles containing OZ might be potentially used for cancer treatment along with other known drug delivery systems based, for example, on lipid nanovesicles [58], leukocyte-mimicking nanovesicles [59], and immunoliposomes [60].

The aim of this research is to obtain OZ-containing depot systems based on P3HB and PHBV in the form of microparticles and casting films and to study their basic physical, chemical, and cytotoxic properties against HeLa cells.

2. Material and Methods

2.1. Material

Microbial P3HB was obtained by the fermentation process: kDa (for emulsification method), kDa (for spray drying method), and PHBV polymer with molar ratio 90 : 10 (P3HB : P3HV) and kDa. Studied polymers and copolymer were produced at the laboratory of Biotechnology of new biomaterials of Siberian Federal University in Krasnoyarsk, RF. OZ was synthesized according to the procedure described in the literature in the Institute of Organic Chemistry, Russian Science Academy [61]. DMSO and PVA were purchased from Sigma-Aldrich (USA). All chemicals were used as received without any further purifications.

2.2. Preparation of OZ-Loaded PHBV Films (OZ-F)

PHBV films containing OZ were prepared by the solution casting method. 250 mg of PHBV was dissolved in chloroform under heating. After that, the solution of OZ in chloroform with a concentration of 1 mg ml^-1 was added to obtain films containing 0.008 wt.%, 0.016 wt.%, and 0.024 wt.% of OZ (23.5, 47, and 70.5 μmol g^-1, respectively). The resulting solution was transferred to a sterile Petri dish and left until complete solvent evaporation (at room temperature for 48 h). As a control, PHBV-based films without OZ were obtained under similar conditions.

2.3. Preparation of OZ-Loaded P3HB Microparticles by Emulsification Method (OZ-MP_EM)

OZ-MP_EM were prepared using water in oil emulsion containing 0.4 g of P3HB, 0.04 g of OZ in 40 ml of dichloromethane (DCM), and 100 ml of 0.5% aqueous PVA solution. The resulting emulsion was mechanically stirred at 5000 rpm for 3 min (Heidolph SilentCrusher M, Germany). After complete evaporation of DCM, OZ-MP_EM were collected by centrifugation at 4000 rpm for 3 min, washed multiple times with deionized water, and then lyophilized.

Unloaded microparticles (MP_EM) were obtained under similar conditions.

2.4. Preparation of OZ-Loaded P3HB Microparticles by Spray Drying Method (OZ-MPSD)

The Büchi spray dryer (BUCHI Laboratory Equipment, Switzerland) OZ-MP_SD was used for the spray drying of P3HB/OZ solution (80 mg of P3HB, 40 mg of OZ dissolved in 80 ml of DCM), with nitrogen as a drying gas, at a flow rate of 4.5 ml min^-1.

Microparticles (MP_SD) without OZ were obtained under similar conditions.

2.5. Characterization of Films

2.5.1. Morphological Analysis

The surface morphology analysis of films was carried out by scanning electron microscopy (SEM) using SU3500 and TM4100 (Hitachi, Japan). In order to determine the average roughness (Sa) of the surface atomic force, microscopy (AFM) (Solver Nano, NT-MDT, Russia) was used.

2.5.2. Determination of Surface Characteristics

Film surface characteristics towards liquids were measured using the Drop Shape Analyzer (KRÜSS, Germany) with distilled water as a polar liquid.

By measuring contact angles (, degrees) (water/air), free surface energy (), free interface energy (), and cohesive force () (erg/cm²) were calculated.

Free surface energy of PHBV films was calculated according to the following equation: where is free water surface tension, 72.8 erg/cm².

Free interface energy (polymer/water) was found from the formula:

The cohesive force was calculated from the formula

2.6. Characterization of Microparticles

2.6.1. Morphological Analysis

The surface morphology analysis of polymer microparticles was carried out as above. A number of samples were sputter coated with platinum using Leica EM ACE200 (Leica Microsystems, Germany) to obtain a good-quality SEM image.

2.6.2. Total Yield of Microparticles

Total yields of microparticles (%) were determined according to where is the mass of microparticles and is the initial mass of P3HB, used in synthesis.

2.6.3. Particle Size and Zeta Potential

Zetasizer Nano ZS (Malvern, UK) was used to determine the average particle size, zeta potential, particle size distribution (PSD), and polydispersity index (PDI). 5 mg of each sample was suspended in deionized water and sonicated at 6 W for 1 min before the measurements.

2.6.4. DSC Analysis

Differential scanning calorimetry, DSC, analysis was carried out to determine the melting behavior of MP_EM and OZ-MP_EM using Mettler Toledo STAR DSC 1 (USA), samples were heated at a rate of 10°C min^-1, and the data were recorded from 20 to 300°C.

2.6.5. Drug Encapsulation Efficiency (for Microparticles) and In Vitro OZ Efflux

The amount of OZ encapsulated in the P3HB microparticles was determined spectrophotometrically using Genesys 10S UV-Vis (Thermo Scientific, USA). Firstly, MP_EM and MP_SD containing OZ were dissolved in DCM. Then, the absorbance (at 268 nm) of each solution was measured, and a calibration curve (pure OZ in DCM) was plotted. The amount of OZ in MPs was determined using the standard curve.

The encapsulation efficiency (EE) was determined according to the formula where is the mass of OZ in microparticles and is the initial mass of OZ.

The investigation of the release of OZ from OZ-MPs was carried out in vitro. Samples of the studied microparticles were placed in sterile centrifuge tubes containing DMSO (2 mg MPs in 1 ml DMSO). Tubes were thermostated at 37°C, with the microparticles precipitated by centrifugation, 11000 rpm, 5 min, at predetermined time intervals. The amount of OZ released was determined by the measurement of absorbance of the supernatant at 268 nm.

To assess the release profile of OZ from OZ-containing films, each film was cut in circles with a diameter of 6 mm. Then, the circles were placed in sterile tubes containing DMSO, and the amount of OZ released during 9 days was measured as mentioned above.

2.7. Cell Culture

HeLa cells, as a model adhesive culture for the estimation of cytotoxic effect, were cultured in Gibco Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, USA) with the addition of fetal bovine serum (HyClone, USA) and standard antibiotic-antimycotic supplement (Sigma-Aldrich, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique was used to evaluate the cytotoxic activity of the obtained depot systems.

For the MTT study, the cell suspension was incubated with the studied systems for 24 hours. Then, MTT (in PBS buffer) was added to all samples; the cell culture plate was then incubated at 37°C for 4 h. The resulting formazan was dissolved in DMSO, and the absorbance was determined at 550 nm using an iMark Microplate Reader (Bio-Rad Laboratories, USA).

Cell viability (%) was calculated according to the equation below: where test is the absorbance of the test sample and control is the absorbance of the control sample.

The Trypan blue assay was used to assess cell death as a result of interaction with depot systems. After coincubation for 24 hours, cells were harvested using 0.25% Trypsin-EDTA (Gibco, USA) and centrifuged; the supernatant was removed and resuspended in DMEM. Trypan blue solution (Gibco, USA) was added to the cell suspension, which was layered on a microscope slide.

The number of living cells was determined in the hemocytometer using the following formula: where is the number of cells in 1 ml, is the number of cells in 5 large squares, and is dilution.

Four replicates were performed for each sample.

To visualize cell viability during interaction with the studied systems, a LIVE/DEAD assay was performed using a ReadyProbes™ double staining kit (Thermo Fisher, USA) in accordance with the manufacturer’s protocol. Images were obtained using the Leica digital microscope (Leica Microsystems GmbH, Germany). Live and dead cells had blue and green fluorescence, respectively. Images were analyzed using NIH ImageJ software.

3. Results and Discussion

In this work, for the first time, two OZ-loaded depot systems based on PHA were developed. The systems obtained were sufficiently stable in model medium and had high drug loading and encapsulation efficiency. We were not absolutely sure about the successful encapsulation of organic peroxides into the PHA matrix, since the interaction of the carboxyl groups of PHAs with peroxides can lead to the decomposition of the peroxide or its rearrangements, such as the rearrangements of Baeyer-Villiger, Criegee, Hock, etc. [62]. According to the obtained absorption spectra of pure OZ and PHAs before and after the encapsulation procedure (not shown), there were no changes noted in the structure of the peaks and their position in the spectra, which was an indication of the absence of any rearrangements or decomposition of molecules.

PHAs have undeniable advantages as a matrix for depot systems, compared to PLGA and other hydrophilic polymers. PHAs are almost inert to hydrolysis and only biodegrade under enzymatic processes in aerobic conditions, without local shifts in the pH of the biological system to the acidic region, as in the case of PLGA. In this work, we studied the release of OZ from microparticles and films containing 7.1% (OZ-MP_SD), 9.7% (OZ-MP_EM), 0.008%, 0.016%, and 0.024% of OZ (wt.%).

3.1. Characterization of Films

For the estimation of the applicability of the approach, we obtained casting films by combining chloroform solutions of two components of the depot system. Resulted films had relatively homogeneous structures. The surface of OZ-F was rough and porous, with pore sizes ranging from 0.5 to 6 μm (Figure 3).

(a)

(b)

(c)

(d)

As a result of the addition of OZ to the PHA, we detected the increasing of the porosity and average roughness of the sample surface, compared with the control, with both microscopy approaches—SEM and AFM. Increasing the ozonide concentration in the film leads to a linear increasing in the average surface roughness (Table 1).

The films’ surface characteristics are presented in Table 1.

Since OZ is encapsulated into the PHBV matrix, all the studied parameters of the obtained films significantly change. When the amount of OZ in the film increases from 0% to 0.008%, the contact angle (water in air) increases by more than 30%: from to degrees, so the resulted film surface becomes more hydrophobic. The free surface energy reaches its maximum at 0.016% of OZ in PHBV film, erg/cm²; the presence of OZ reduces the free interface energy of the PHBV-OZ composite film. The cohesion forces are also increased, when OZ is added, by about 5-11%.

Thus, with an increase of the ozonide concentration in the PHBV film, both its morphological, average roughness, and physical characteristics: water contact angle and free surface energy, free interface energy, and cohesion forces change. These results make it possible to predict the properties of these depot systems related to biological objects—water-containing medium, tissues, and cells. An increasing of cohesive forces can be regarded as a positive factor contributing to the tight attachment of cellular elements.

3.2. Characterization of Microparticles

OZ-MP have a uniform spherical shape. OZ-MP_SD were found larger and smoother than OZ-MP_EM. It was also noted that OZ-MP_EM (Figure 4(b)) have minor surface defects.

(a)

(b)

(c)

(d)

The encapsulation of OZ into microparticles leads to the insignificant increasing in particle size: the average diameter of OZ-MP_EM and OZ-MP_SD was μm and μm, respectively, while the average diameter of MP_EM was μm and the average size of MP_SD was μm.

The results also indicate that ζ-potential markedly decreases when OZ is encapsulated into both types of microparticles. The value of zeta potential of OZ-MP was for EM and mV for SD, respectively (Table 2). The zeta potential of MP_EM was and mV for MP_SD. In general, the morphology, size, and zeta potential of the microparticles obtained in this research are typical for PHA-based depot systems. Thus, encapsulation of ceftriaxone into the P3HB matrix (Murueva et al. [63]) also leads to increasing the microparticle size.

The size of microparticles was μm for EM and μm for SD, compared to μm (EM) and μm (SD) for empty particles obtained under similar conditions. Zeta potential for ceftriaxone-loaded MP_EM and MP_SD was mV and mV, respectively (compared to mV and mV for unloaded microspheres). Shershneva et al. [52] reported that the average diameter of P3HB-MP_SD containing paclitaxel was μm, and zeta potential was mV, which is slightly lower than the same parameters of blank P3HB-MP_SD: the average diameter was μm, and zeta potential was mV.

The yield of microparticles was excellent for OZ-MP_EM, , and for OZ-MP_SD.

The encapsulation efficiency of OZ was and in MP_EM and MP_SD, respectively. EE of MP_SD is expectedly lower compared to EE of MP_EM. It can be explained by the peculiarities of the spray drying method [63].

3.2.1. Thermal Characteristics

According to the DSC results, OZ-MP_EM have a lower melting point (°C) and crystallization temperature (117.4°C) compared to MP_EM (176°C and 123.6°C, respectively). This is consistent with the results of Senhorini et al. [64], who noted a decrease in of PHBV (8.7% of P3HB) MP after encapsulation of andiroba oil, which has anti-inflammatory and insecticidal properties. Bidone et al. [65] and Volova et al. [66] reported that there is also a decrease in the melting point of polymer compositions based on P3HB containing ibuprofen (by 10°C) and metribuzin (by 2°C), respectively.

As a result, OZ provides greater fusibility of the microparticle-based depot system, which can potentially lead to faster biodegradation.

3.2.2. OZ Release from MPs and Fs In Vitro

OZ release profiles from MP_EM and MP_SD are presented in Figure 5.

A significant drug release—60% for OZ-MP_EM and 48% for OZ-MP_SD—is achieved in 24 hours, and the full release of OZ occurs in 144 and 216 hours, respectively.

It was found that the OZ release from OZ-MP_EM and OZ-MP_SD depends on the sizes of microparticles and their surface structure having defects as pores and microcracks. OZ-MP_SD have a smoother surface and larger sizes compared to OZ-MP_EM, so the release rate from these microparticles is slower.

Each kinetic curve is characterized by two regions: the linear (0–24 h) and nonlinear (48–312 h) stages of OZ release from microparticles. To determine the drug release mechanism according to the Higuchi model, the diffusion coefficients were calculated at each of these regions.

In case of a short desorption time of OZ at the initial stage, Fick’s second law (according to Higuchi model [67]) can be written as follows: where is the mass of OZ desorbed at time , is the mass of OZ desorbed at an infinite time (), and is matrix thickness.

Since the MP has a spherical shape, the matrix thickness () is replaced by the particle diameter (). After linearization of the previous equation,

The graphical solution of Equation (9) (Figure 6) allows us to determine the diffusion coefficient of OZ at the initial stage of the kinetic curve (). is equal to 6.1·10^-11 cm²/s for OZ-MP_EM and 2.2·10^-9 cm²/s for OZ-MP_SD.

To determine the diffusion coefficient at the second stage () (), the following approximation is used:

The graphical solution of Equation (10) in semilog coordinates () (Figure 7) allows us to calculate :

for OZ-MP_EM is 2.7·10^-11 cm²/s and for OZ-MP_SD is 0.5·10^-9 cm²/s.

A significant decrease of for both types of microparticles indicates that the release mechanism of OZ changes.

Livshits et al. [68] obtained similar diffusion coefficients for microparticles of different sizes. For particles with an average size of 0.4 μm, the diffusion coefficients of dipyridamole into the release medium were 0.1·10^-11 cm²/s () and 0.08·10^-11 cm²/s () and for particles with an average size of 1.9 μm were 1.5·10^-11 cm²/s () and 2.0·10^-11 cm²/s ().

OZ release profiles from OZ-F are presented in Figure 8.

During the first 24 hours, significant drug release was noted: 22% for OZ-F (0.008 wt.%), 29% for OZ-F (0.016 wt.%), and 33% for OZ-F (0.024 wt.%).

Depot systems based on OZ-containing PHBV films are active for at least 9 days during the observation period. The amount of released OZ reaches 60% of encapsulated, for OZ-F containing 70.5 μmol g^-1 (0.024 wt.%) of OZ, 49% for OZ-F containing 47 μmol g^-1 (0.016 wt.%) of OZ, and 40% for OZ-F containing 23.5 μmol g^-1 (0.008 wt.%) of OZ.

The undoubted advantage of PHAs-based depot systems is the long period of their biological activity. These systems are active for several weeks and probably even months [69, 70], according to the dynamics of PHA degradation temps, which will be especially important in agriculture, for use in soils and in fields, during germination periods.

3.3. In Vitro Cytotoxicity

During the cultivation of HeLa in the presence of OZ-containing samples, it was noted that all depot systems inhibit cell growth and proliferation. The cytotoxicity directly depends on the concentration of OZ. Free OZ, suspended in water, showed pronounced cytotoxicity against HeLa cells.

The presence of OZ in PHA films significantly reduced cell viability (%). A decrease in the number of viable cells (Table 3) was observed in the case of the film with the highest content of OZ (70.5 μmol g^-1), when only 2% of viable cells were noted.

OZ-MP_EM and OZ-MP_SD are slightly less active against HeLa cells (Table 4) apparently due to the not as close sample-cell contact as in the case of depot films.

The results of the LIVE/DEAD assay for films are presented in Figure 9.

(a)

(b)

(c)

(d)

The results of the LIVE/DEAD assay for microparticles and free OZ are presented in Figure 10.

(a)

(b)

(c)

(d)

(e)

As expected, it was noted that with the increase of OZ concentration in the film obtained, the number of viable cells significantly decreases, while the active concentrations of OZ are in the range of extremely low values from 47 to 70.5 μmol g^-1. At a concentration of 23.5 μmol g^-1, the HeLa cell viability was approximately 20%, which also confirms the high activity of the studied ozonide. Thus, the characteristics of the obtained OZ depot systems provide a basis for scaling up the study of the cytotoxic effects of OZ when it is encapsulated in the PHA matrix with the further development of anticancer, antiparasitic, antiviral, and other depot forms.

4. Conclusion

For the first time, two types of depot systems containing new organic peroxide—casting films and microparticles—based on microbial PHAs were synthesized. The addition of 1,2,4-trioxolane insignificantly changes the main physical characteristics of the PHA matrix: the decrease of melting point and crystallization temperature was noted. Microparticles loaded with OZ were obtained using two methods of micronization of polymer solutions: emulsification and spray drying, with the inclusion of OZ up to 9.7 mass % and its complete sustained release in vitro during 312 h. Casting films containing 0.008%, 0.016%, and 0.024% of OZ caused a significant decrease in the number of living cells of the model cancer culture, which was the result of the high activity of the deposited compound. However, the limitations of the obtained systems are the difficulty in assessing the process of biodegradation of films and particles at this stage, as well as the relatively high sizes of microparticles. Reducing the particle size and increasing the zeta potential can potentially increase their aggregative stability in the studied media. Thus, depot systems containing 1,2,4-trioxolane based on biodegradable natural polymers have been developed.

Data Availability

All data included in this study are available upon request by contacting with the corresponding author.

Conflicts of Interest

There are no conflicts to declare.

Acknowledgments

This study was financially supported by the project “Agropreparations of the new generation: a strategy of construction and realization” (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, “On measures designed to attract leading scientists to the Russian institutions of higher learning.”

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Copyright © 2022 Sergei Y. Lipaikin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Advances in Polymer Technology

Development of Biodegradable Delivery Systems Containing Novel 1,2,4-Trioxolane Based on Bacterial Polyhydroxyalkanoates

Abstract

1. Introduction

2. Material and Methods

2.1. Material

2.2. Preparation of OZ-Loaded PHBV Films (OZ-F)

2.3. Preparation of OZ-Loaded P3HB Microparticles by Emulsification Method (OZ-MPEM)

2.4. Preparation of OZ-Loaded P3HB Microparticles by Spray Drying Method (OZ-MPSD)

2.5. Characterization of Films

2.5.1. Morphological Analysis

2.5.2. Determination of Surface Characteristics

2.6. Characterization of Microparticles

2.6.1. Morphological Analysis

2.6.2. Total Yield of Microparticles

2.6.3. Particle Size and Zeta Potential

2.6.4. DSC Analysis

2.6.5. Drug Encapsulation Efficiency (for Microparticles) and In Vitro OZ Efflux

2.7. Cell Culture

3. Results and Discussion

3.1. Characterization of Films

3.2. Characterization of Microparticles

3.2.1. Thermal Characteristics

3.2.2. OZ Release from MPs and Fs In Vitro

3.3. In Vitro Cytotoxicity

4. Conclusion

Data Availability

Conflicts of Interest

Acknowledgments

References

Copyright

2.3. Preparation of OZ-Loaded P3HB Microparticles by Emulsification Method (OZ-MP_EM)