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This Article Full Text Full Text (PDF) All Versions of this Article: 290/2/F364    most recent 00110.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Related articles in AJP - Renal Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Camargo de Andrade, M. C. Articles by Casarini, D. E. Search for Related Content PubMed PubMed Citation Articles by Camargo de Andrade, M. C. Articles by Casarini, D. E.

Expression and localization of N-domain ANG I-converting enzymes in mesangial cells in culture from spontaneously hypertensive rats

¹Disciplina de Nefrologia, Departamento de Medicina, ²Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia, e Parasitologia, and ³Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, Brazil

Submitted 21 March 2005 ; accepted in final form 28 July 2005

The angiotensin-converting enzyme (ACE) profile in urine of hypertensive patients and spontaneously hypertensive rats (SHR; 90- and 65-kDa N-domain ACEs) is different from that of healthy subjects and Wistar rats (190 and 65 kDa). In addition, four ACE isoforms were purified from mesangial cells (MC) of Wistar rats in the intracellular compartment (130 and 68 kDa) and as secreted forms (130 and 60 kDa). We decided to characterize ACE forms from SHR MC in culture. Analysis of the ACE gene showed that SHR MC are able to express ACE mRNA. The concentrated medium and cell homogenate were separately purified by gel filtration and then subjected to lisinopril-Sepharose chromatography. The molecular masses of purified enzymes, 90 kDa for ACEm1A and 65 kDa for ACEm2A (secreted enzymes) and 90 kDa for ACEInth1A and 65 kDa for ACEInth2A (intracellular), were different from those of Wistar MC. The purified enzymes are Cl^– dependent, inhibited by enalaprilat and captopril, and able to hydrolyze AcSDKP. Immunofluorescence and cell fractionation followed by Western blotting showed predominant immunoreaction of the 9B9 antiserum for N-domain ACE in the nuclei. The N-domain ACE was localized in the glomerulus from Wistar rats and SHR. ANG II and ANG-(1–7) were localized in the cell cytoplasm and nuclei. The 90-kDa N-domain ACE, described recently as a possible genetic marker of hypertension, was found inside the cell nuclei of SHR MC colocalized with ANG II and ANG-(1–7). The presence of ANG II in the cell nuclei could suggest an important role for this peptide in the transcription of new genes.

nuclei; hypertension; mesangial cell; regulation of gene expression

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AJP - Renal 2006 291: F921. [Full Text]  

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