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CARDIAC, RENAL, AND RESPIRATORY INTEGRATION

A comparison of adrenergic stress responses in three tropical teleosts exposed to acute hypoxia

³Department of Physiological Sciences, Laboratory of Zoophysiology and Comparative Biochemistry, Federal University of São Carlos, São Carlos, Brazil; ¹Ottawa-Carleton Institute of Biology, Ottawa, Ontario K1N 6NS; ²Department of Life Sciences, University of Toronto at Scarborough, Toronto, Ontario M1C 1AU; and ⁴Department of Zoology, University of British Columbia, Vancouver, British Columbia, V6T 1Z4 Canada

Submitted 9 December 2003 ; accepted in final form 17 March 2004

Experiments were performed to assess the afferent and efferent limbs of the hypoxia-mediated humoral adrenergic stress response in selected hypoxia-tolerant tropical fishes that routinely experience environmental O₂ depletion. Plasma catecholamine (Cat) levels and blood respiratory status were measured during acute aquatic hypoxia [water PO₂ (Pw_O2) = 10–60 mmHg] in three teleost species, the obligate water breathers Hoplias malabaricus (traira) and Piaractus mesopotamicus (pacu) and the facultative air breather Hoplerythrinus unitaeniatus (jeju). Traira displayed a significant increase in plasma Cat levels (from 1.3 ± 0.4 to 23.3 ± 15.1 nmol/l) at Pw_O2 levels below 20 mmHg, whereas circulating Cat levels were unaltered in pacu at all levels of hypoxia. In jeju denied access to air, plasma Cat levels were increased markedly to a maximum mean value of 53.6 ± 19.1 nmol/l as Pw_O2 was lowered below 40 mmHg. In traira and jeju, Cat release into the circulation occurred at abrupt thresholds corresponding to arterial PO₂ (Pa_O2) values of approximately 8.5–12.5 mmHg. A comparison of in vivo blood O₂ equilibration curves revealed low and similar P₅₀ values (i.e., Pa_O2 at 50% Hb-O₂ saturation) among the three species (7.7–11.3 mmHg). Thus Cat release in traira and jeju occurred as blood O₂ concentration was reduced to approximately 50–60% of the normoxic value. Intravascular injections of nicotine (600 nmol/kg) elicited pronounced increases in plasma Cat levels in traira and jeju but not in pacu. Thus the lack of Cat release during hypoxia in pacu may reflect an inoperative or absent humoral adrenergic stress response in this species. When allowed access to air, jeju did not release Cats into the circulation at any level of aquatic hypoxia. The likeliest explanation for the absence of Cat release in these fish was that air breathing, initiated by aquatic hypoxia, prevented Pa_O2 values from falling to the critical threshold required for Cat secretion. The ventilatory responses to hypoxia in each species were similar, consisting generally of increases in both frequency and amplitude. These responses were not synchronized with or influenced by plasma Cat levels. Thus the acute humoral adrenergic stress response does not appear to stimulate ventilation during acute hypoxia in these tropical species.

air breathing; cortisol