Xiaoxia Zhang
ABSTRACT
The extract of Apium graveolens L. root (AGRE) in Xinjiang has exhibited a lipid-lowering effects against development of hyperlipidemia in rat models. however, its underlying molecular mechanisms are largely unclear. The effects of AGRE and its major constituents were investigated on hepatic steatosis in L02 and HepG2 cells induced by free fatty acids (FFA). The results show that the contents of apigenin (AGN) and apiin (AIN) in AGRE were 11.69 μg/mg and 28.50 μg/mg, respectively. AGN, AIN and AGRE significantly reduced the content of triglyceride (TG) (P<0.05) and significantly decreased the levels of inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) (P<0.05). The AGN, AIN and AGRE groups displayed elevated expression of adiponectin (ADPN) . At the protein level, AMP-activated protein kinase α (AMPKα ) α and acetyl-CoA carboxylase (ACC) phosphorylation increased (P<0.05), whilst sterol regulatory element-binding protein-1c (SREBP-1c) expression decreased (P<0.01). Therefore, AGRE and its major constituents have promising regulation effect of FFA metabolism and attenuation of lipid accumulation through AMPK/SREBP-1c signaling.
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