Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system

Saki Imai; Zhiqing Li; Kazuhiro Iiyama; Yoshitaka Miyagawa; Masashi Toyoda; Akihiro Umezawa; Hiroaki Mon; Takahiro Kusakabe; Kaito Yoshimura; Jae Man Lee

doi:10.11416/jibs.82.2_039

Regular Articles

Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system

Saki Imai, Zhiqing Li, Kazuhiro Iiyama, Yoshitaka Miyagawa, Masashi Toyoda, Akihiro Umezawa, Hiroaki Mon, Takahiro Kusakabe, Kaito Yoshimura, Jae Man Lee

Author information

Saki Imai
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
Zhiqing Li
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
Kazuhiro Iiyama
Laboratory of Insect Pathology, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
Yoshitaka Miyagawa
National Research Institute for Child Health and Development
Masashi Toyoda
National Research Institute for Child Health and Development
Akihiro Umezawa
National Research Institute for Child Health and Development
Hiroaki Mon
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
Takahiro Kusakabe
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
Kaito Yoshimura
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
Jae Man Lee
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences

Keywords: bone morphogenetic protein 4, silkworm-baculovirus expression system, collagen binding domain, BmNPV

JOURNAL FREE ACCESS

2013 Volume 82 Issue 2 Pages 2_039-2_044

DOI https://doi.org/10.11416/jibs.82.2_039

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Abstract

Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fibronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22mg/ml (0.1mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purified rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.

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