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Sphingosine and Other Amine-Containing Compounds Induce Rapid NOX-Independent NETosis

  • NANOBIOMEDICINE AND NANOPHARMACEUTICALS
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Abstract

NETosis is a form of neutrophil death in which DNA, chromatin, and bactericidal proteins of granules are secreted into the extracellular space and form a network in which pathogenic microorganisms die. The induction of NETosis by small molecular substances (sphingosine, fingolimod, stearylamine, stearic acid, ceramide) is studied. Fluorescence microscopy reveals that sphingosine induces NETosis in a primary culture of human neutrophils within 60 min. Compared with the classic NETosis inducer, phorbol 12-myristate 13-acetate, sphingosine-induced NETosis can be classified as “rapid.” Stearylamine and fingolimod are significantly less active. Ceramide and stearic acid do not enhance NETosis compared with the control. It is found that blocking NADPH oxidase with apocynin and DPI and inhibiting protein kinase C with chelerythrine do not affect the formation of traps under the action of sphingosine. Using the method of chemiluminescence, it is revealed that sphingosine and other studied compounds suppress the oxidative burst of neutrophils induced by phorbol 12-myristate 13-acetate and latex. The results obtained allow us to conclude that sphingosine, stearylamine, and fingolimod induce the development of such a type of NETosis, which is not associated with the activation of NADPH oxidase and develops according to a mechanism different from that of NOX-dependent NETosis development.

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REFERENCES

  1. V. Brinkmann, U. Reichard, C. Goosmann, et al., Science 303, 1532 (2004). https://doi.org/10.1126/science.109238

    Article  CAS  PubMed  ADS  Google Scholar 

  2. M. Worku, D. Rehrah, H. D. Ismail, et al., Int. J. Mol. Sci. 22, 8046 (2021). https://doi.org/10.3390/ijms22158046

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. T. A. Fuchs, U. Abed, C. Goosmann, et al., J. Cell Biol. 176, 231 (2007). https://doi.org/10.1083/jcb.200606027

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. H. Parker, M. Dragunow, M. B. Hampton, et al., J. Leukocyte Biol. 92, 841 (2012). https://doi.org/10.1189/jlb.1211601

    Article  CAS  PubMed  Google Scholar 

  5. V. Papayannopoulos, K. D. Metzler, A. Hakkim, et al., J. Cell Biol. 191, 677 (2010). https://doi.org/10.1083/jcb.201006052

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. O. Goldmann and E. Medina, Front. Immunol. 3, 420 (2013). https://doi.org/10.3389/fimmu.2012.00420

    Article  PubMed  PubMed Central  Google Scholar 

  7. Q. Remijsen, T. Berghe, E. Wirawan, et al., Cell. Res. 21, 290 (2011). https://doi.org/10.1038/cr.2010.150

    Article  CAS  PubMed  Google Scholar 

  8. F. H. Pilsczek, D. Salina, K. K. H. Poon, et al., J. Immunol. 185, 7413 (2010). https://doi.org/10.4049/jimmunol.1000675

    Article  CAS  PubMed  Google Scholar 

  9. C. Díaz-Godínez, Z. Fonseca, M. Néquiz, et al., Front. Cell. Infect. Microbiol. 8, 184 (2018). https://doi.org/10.3389/fcimb.2018.00184

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. D. N. Douda, M. A. Khan, H. Grasemann, et al., Proc. Natl. Acad. Sci. U.S.A. 112, 2817 (2015). https://doi.org/10.1073/pnas.1414055112

    Article  CAS  PubMed  PubMed Central  ADS  Google Scholar 

  11. N. Vorobjeva, I. Galkin, O. Pletjushkina, et al., Biochim. Biophys. Acta, Mol. Basis Dis. 1866, 165664 (2020). https://doi.org/10.1016/j.bbadis.2020.165664

  12. Y. Arai, Y. Nishinaka, T. Arai, et al., Biochem. Biophys. Res. Commun. 443, 556 (2014). https://doi.org/10.1016/j.bbrc.2013.12.007

    Article  CAS  PubMed  Google Scholar 

  13. K. Chen, H. Nishi, R. Travers, et al., Blood 120, 4421 (2012). https://doi.org/10.1182/blood-2011-12-401133

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. N. Yu. Lotosh, S. O. Alyaseva, I. K. Malashenkova, et al., Biol. Membr. 35, 448 (2018).

    Google Scholar 

  15. N. Yu. Lotosh, A. A. Selishcheva, and N. V. Vorobyeva, Ross. Immunol. Zh. 13, 855 (2019). https://doi.org/10.31857/S102872210006650-0

    Article  Google Scholar 

  16. N. Yu. Lotosh, S. O. Alyaseva, R. G. Vasilov, et al., Cell Tissue Biol. 13, 366–375 (2019). https://doi.org/10.1134/S0041377119040035

    Article  Google Scholar 

  17. M. A. Khan, C. Pace-Asciak, J. M. Al-Hassan, et al., Biomolecules 8, 144 (2018). https://doi.org/10.3390/biom8040144

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Z. Wang, H. Fan, R. Xie, et al., Acta Haematol. 134, 49 (2015). https://doi.org/10.1159/000369291

    Article  CAS  PubMed  Google Scholar 

  19. L. Zhang, H. Gao, L. Yang, et al., Arch. Biochem. Biophys. 711, 109015 (2021). https://doi.org/10.1016/j.abb.2021.109015

  20. Y. A. Hannun and L. M. Obeid, Nat. Rev. Mol. Cell Biol. 19, 175 (2018). https://doi.org/10.1038/nrm.2017.107

    Article  CAS  PubMed  Google Scholar 

  21. Y. A. Hannun, C. R. Loomis, A. H. Merrill, et al., J. Biol. Chem. 261, 12604 (1986). https://doi.org/10.1016/S0021-9258(18)67133-9

    Article  CAS  PubMed  Google Scholar 

  22. A. M. Bryan and M. Del Poeta, Cell. Microbiol. 20, e12836 (2018). https://doi.org/10.1111/cmi.12836

  23. M. P. Espaillat, R. R. Kew, and L. M. Obeid, Adv. Biol. Regul. 63, 140 (2017). https://doi.org/10.1016/j.jbior.2016.11.001

    Article  CAS  PubMed  Google Scholar 

  24. M. A. Van der Linden, G. H. Westerlaken, M. Van der Vlist, et al., Sci. Rep. 26, 6529 (2017). https://doi.org/10.1038/s41598-017-06901-w

    Article  CAS  ADS  Google Scholar 

  25. N. C. Rochael, A. B. Guimarães-Costa, M. T. C. Nascimento, et al., Sci. Rep. 5, 1 (2015). https://doi.org/10.1038/srep18302

    Article  CAS  Google Scholar 

  26. L. R. De Chatelet, C. J. Lees, C. E. Walsh, et al., Infect. Immunol. 38, 969 (1982). https://doi.org/10.1128/iai.38.3.969-974.1982

    Article  CAS  Google Scholar 

  27. L. Abdel Hadi, C. Di Vito, and L. Riboni, Mediators Inflamm. 2016, 13 (2016). https://doi.org/10.1155/2016/3827684

    Article  CAS  Google Scholar 

  28. N. J. Pyne, M. McNaughton, S. Boomkamp, et al., Adv. Biol. Regul. 60, 151 (2016). https://doi.org/10.1016/j.jbior.2015.09.001

    Article  CAS  PubMed  Google Scholar 

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ACKNOWLEDGMENTS

The authors are grateful to the staff of the Medical Scientific Clinical Diagnostic Center KK NBICS-PT for their participation in the collection of biomaterial.

Funding

This work was supported by the National Research Center Kurchatov Institute (thematic plan 5p.5.3 “Development of universal platforms for the express diagnostics of infectious diseases based on modern genetic technologies,” state-registration topic number 121031600197-5 Moscow State University).

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Authors and Affiliations

  1. National Research Center “Kurchatov Institute”, 123182, Moscow, Russia

    A. A. Voitenok, N. Yu. Lotosh, E. A. Kulikov, A. G. Rogov, R. G. Vasilov & A. A. Selishcheva

  2. Faculty of Biology, Moscow State University, 119991, Moscow, Russia

    A. A. Selishcheva

  3. MIREA–Russian Technological University, 119454, Moscow, Russia

    A. A. Voitenok

Authors
  1. A. A. Voitenok
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  2. N. Yu. Lotosh
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  3. E. A. Kulikov
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  4. A. G. Rogov
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  5. R. G. Vasilov
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  6. A. A. Selishcheva
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Corresponding author

Correspondence to N. Yu. Lotosh.

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CONFLICT OF INTEREST

The authors of this work declare that they have no conflicts of interest.

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

Venous blood was obtained from healthy volunteers who signed informed consent.All manipulations were performed according to protocol no. NG-1/01.13 dated March 11, 2021, of the Local Ethics Committee for Biomedical Research, National Research Center “Kurchatov Institute.”

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Translated by D. Novikova

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Voitenok, A.A., Lotosh, N.Y., Kulikov, E.A. et al. Sphingosine and Other Amine-Containing Compounds Induce Rapid NOX-Independent NETosis. Nanotechnol Russia 18, 952–959 (2023). https://doi.org/10.1134/S2635167623601080

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  • DOI: https://doi.org/10.1134/S2635167623601080

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