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AEDG Peptide Prevents Oxidative Stress in the Model of Induced Aging of Skin Fibroblasts

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Abstract

Ultraviolet (UV) radiation is one of the main environmental factors leading to oxidative stress and accelerated skin aging. AEDG peptide, a regulator of pineal gland functions, has demonstrated geroprotective and antioxidant effects in in vivo and in vitro studies. The aim of this research was to evaluate the effect of AEDG peptide on the expression of genes encoding enzymes of the antioxidant system (NQO1, SOD1, CATALASE, and TRXR) in human skin fibroblasts in a model of accelerated aging induced by UV radiation. The expression of SOD1 and TXNRD1 genes during UV-induced aging of dermal fibroblasts increases by 2 and 1.7 times, respectively. This can be considered as a defense mechanism against oxidative stress caused by UV radiation. Photoaging does not influence the expression of the NQO1 and CATALASE genes in the culture of skin fibroblasts. AEDG peptide promotes the expression of the SOD-1, NQO1, and CATALASE genes in dermal fibroblasts exposed to UV radiation by 2.7, 2.6, and 3.2 times, respectively. AEDG peptide can stimulate the expression of these genes via the Keap1/Nrf2 signaling pathway. AEDG peptide might be potentially effective in preventing the accelerated aging of dermal fibroblasts.

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Authors and Affiliations

  1. St. Petersburg Institute of Bioregulation and Gerontology, 197110, St. Petersburg, Russia

    E. O. Gutop, N. S. Linkova, E. O. Kozhevnikova, N. V. Fridman, O. M. Ivko & V. Kh. Khavinson

  2. Belgorod State National Research University, 308015, Belgorod, Russia

    N. S. Linkova

  3. Pavlov Institute of Physiology, Russian Academy of Sciences, 199034, St. Petersburg, Russia

    V. Kh. Khavinson

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  1. E. O. Gutop
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  2. N. S. Linkova
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  3. E. O. Kozhevnikova
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  4. N. V. Fridman
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  5. O. M. Ivko
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  6. V. Kh. Khavinson
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Correspondence to V. Kh. Khavinson.

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Statement of compliance with standards of research involving humans as subjects. Patient signed an informed consent. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Translated by E. Sherstyuk

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Gutop, E.O., Linkova, N.S., Kozhevnikova, E.O. et al. AEDG Peptide Prevents Oxidative Stress in the Model of Induced Aging of Skin Fibroblasts. Adv Gerontol 12, 143–148 (2022). https://doi.org/10.1134/S2079057022020096

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  • DOI: https://doi.org/10.1134/S2079057022020096

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