Abstract
Chlorpromazine causes distinct defects in normal development of early cleaving embryos of gastropod pond snail (Lymnaea stagnalis L.) and teleost Eurasian weather loach (Misgurnus fossilis L.): a decrease in blastomere contact surfaces following the rounding in gastropod embryo and a reduction of cleaving blastodisk surface in teleost. Right after the application of chlorpromazine fluorescence is visible as bright spots on the surface of blastomeres. Then spherical vesicles appear within the whole volume of the cells. In loach, association of chlorpromazine with the plasma membrane was observed in blastodisk but not in the yolk cell membrane. Electron microscopy has shown that chlorpromazine induces folding of the cell membrane. Experiments with fluorescent dextran probe demonstrate that chlorpromazine modifies the rate and character of the dextran uptake. Our results indicate that chlorpromazine binds to specific sites of plasmalemma and stimulates endocytosis. Patterns of chlorpromazine binding to the membrane and its effects on endocytosis are similar in teleost and gastropod.
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Original Russian Text © E.G. Ivashkin, E.E. Voronezhskaya, 2011, published in Biologicheskie Membrany, 2011, Vol. 28, No. 4, pp. 290–297.
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Ivashkin, E.G., Voronezhskaya, E.E. Chlorpromazine-induced changes of endocytosis in blastomeres of the embryos of pond snail Lymnaea stagnalis L. and Eurasian weather loach Misgurnus fossilis L.. Biochem. Moscow Suppl. Ser. A 5, 242–248 (2011). https://doi.org/10.1134/S1990747811040052
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DOI: https://doi.org/10.1134/S1990747811040052