Abstract
The method of confocal microscopy was applied to pH changes recorded in the apoplast of the stem of a pumpkin seedling during the generation of the action potential and variation potential. To record the change in pH, the fluorescent probe FITC-dextran was used. An analysis of the obtained fluorescent images and the determination of the fluorescence spectra showed that FITC-dextran is located in the stemcell walls. The propagation of the action and variation potentials is accompanied by a transient increase in the probe fluorescence intensity, which indicates the alkalization of cell walls. This transient alkalization is suggested to be due to the temporary inactivation of electrogenic H+-pump in the plasma membrane.
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Abbreviations
- VP:
-
variation potential
- AP:
-
action potential
- I:
-
intensity of fluorescence
- ΔU:
-
extracellularly recorded potential difference
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Original Russian Text © V.A. Vodeneev, E.K. Akinchits, L.A. Orlova, V.S. Sukhov, I.V. Balalaeva, 2010, published in Tsitologiya, Vol. 52, No. 5, 2010, pp. 549–554.
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Vodeneev, V.A., Akinchits, E.K., Orlova, L.A. et al. Recording changes in extracellular pH via confocal microscopy during generation of excitation potentials in higher plants. Cell Tiss. Biol. 4, 471–475 (2010). https://doi.org/10.1134/S1990519X1005010X
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DOI: https://doi.org/10.1134/S1990519X1005010X