Abstract
The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.
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ACKNOWLEDGMENTS
We thank M. Petrichenkov from the Budker Institute of Nuclear Physics SB RAS for help with irradiation of onion seedlings.
Funding
Work on assessing DNA damage in onion seedling nuclei was performed using the equipment of the Core Facility for Microscopic Analysis of Biological Objects, Siberian Branch, Russian Academy of Sciences, funded under the research at the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences. This work was supported in part by the Russian Foundation for Basic Research (project no. 18-44-240001).
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Translated by M. Batrukova
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Bolsunovsky, A.Y., Dementyev, D.V., Frolova, T.S. et al. Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings. Dokl Biochem Biophys 489, 362–366 (2019). https://doi.org/10.1134/S1607672919060024
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DOI: https://doi.org/10.1134/S1607672919060024