Abstract
The characteristics of the formation of the superoxide radical anion (\(\rm{O}_2^{\bullet-}\)) and hydrogen peroxide by xanthine oxidases isolated from microorganisms and from cow’s milk were investigated. The increase in pH led to an increase in the rate of xanthine oxidation with oxygen by both xanthine oxidases. The functioning of xanthine oxidase from milk along with the two-electron reduction of O2 to H2O2 carries through the one-electron reduction of O2 to \(\rm{O}_2^{\bullet-}\), and the rate and the fraction of generation of \(\rm{O}_2^{\bullet-}\) increased with increasing pH. Under operation of the microbial xanthine oxidase, the \(\rm{O}_2^{\bullet-}\) radical was not detected in the medium. The results suggest a difference in the operation of active centers of enzyme from different sources.
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Original Russian Text © A.S. Gorbunova, M.M. Borisova-Mubarakshina, I.A. Naydov, S.S. Osochuk, B.N. Ivanov, 2018, published in Doklady Akademii Nauk, 2018, Vol. 483, No. 5.
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Gorbunova, A.S., Borisova-Mubarakshina, M.M., Naydov, I.A. et al. Comparison of the Functional Activities of Xanthine Oxidases Isolated from Microorganisms and from Cow’s Milk. Dokl Biochem Biophys 483, 355–358 (2018). https://doi.org/10.1134/S1607672918060170
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DOI: https://doi.org/10.1134/S1607672918060170