Abstract
A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Khaydukov, E.V., Mironova, K.E., Semchishen, V.A., Generalova, A.N., Nechaev, A.V., Khochenkov, D.A., Stepanova, E.V., Lebedev, O.I., Zvyagin, A.V., Deyev, S.M., and Panchenko, V.Ya., Sci. Rep., 2016, vol. 6, p. 35103.
Liang, L., Lu, Y., Zhang, R., Care, A., Ortega, T.A., Deyev, S.M., Qian, Y., and Zvyagin, A.V., Acta Biomater., 2017, vol. 51, pp. 461–470
Zelepukin, I.V., Shipunova, V.O., Mirkasymov, A.B., Nikitin, P.I., Nikitin, M.P., and Deyev, S.M., Acta Naturae, 2017, vol. 9, no. 4, pp. 58–65
Shipunova, V.O., Nikitin, M.P., Zelepukhin, I.V., Nikitin, P.I., and Deyev, S.M., Dokl. Biochem. Biophys., 2015, vol. 464, pp. 315–318
Arakaki, A., Webb, J., and Matsunaga, T., J. Biol. Chem., 2003, vol. 278, no. 10, pp. 8745–8750
Xie, J., Chen, K., and Chen, X., Nano Res., 2009, vol. 2, pp. 261–278
Rawlings, A.E., Bramble, J.P., Hounslow, A.M., Williamson, M.P., Monnington, A.E., Cooke, D.J., and Staniland, S.S., Chem.-Eur. J., 2016, vol. 22, no. 23, pp. 7885–7894
Deyev, S.M. and Lebedenko, E.N., BioEssays, 2008, vol. 30, no. 9, pp. 904–918
Deyev, S.M., Waibel, R., Lebedenko, E.N., Schubiger, A.P., and Plückthun, A., Nat. Biotechnol., 2003, vol. 21, pp. 1486–1492
Aghayeva, U.F., Nikitin, M.P., Lukash, S.V., and Deyev, S.M., ACS Nano, 2013, vol. 7, no. 2, pp. 950–961
Steiner, D., Forrer, P., and Plückthun, A., J. Mol. Biol., 2008, vol. 382, no. 5, pp. 1211–1227
Shipunova, V.O., Nikitin, M.P., Nikitin, P.I., and Deyev, S.M., Nanoscale, 2016, vol. 8, no. 25, pp. 12764–12772
Nikitin, M.P., Shipunova, V.O., Deyev, S.M., and Nikitin, P.I., Nat. Nanotechnol., 2014, vol. 9, no. 9, pp. 716–722
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © P.A. Kotelnikova, V.O. Shipunova, U.F. Aghayeva, O.A. Stremovskiy, M.P. Nikitin, I.A. Novikov, A.A. Schulga, S.M. Deyev, R.V. Petrov, 2018, published in Doklady Akademii Nauk, 2018, Vol. 481, No. 2.
Rights and permissions
About this article
Cite this article
Kotelnikova, P.A., Shipunova, V.O., Aghayeva, U.F. et al. Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells. Dokl Biochem Biophys 481, 198–200 (2018). https://doi.org/10.1134/S1607672918040051
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672918040051