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Kunitz-type peptides of the sea anemone Heteractis crispa: Potential anti-inflammatory compounds

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Abstract

Antihistamine activity of recombinant peptides, Kunitz-type serine protease inhibitors of the sea anemone Heteractis crispa, was studied. It was shown that the peptides rHCGS1.19 and rHCGS1.36 at a concentration 10 μM inhibit an increase in the calcium ion concentration in macrophages elicited by histamine at 62.2 and 84.0%, respectively. The anti-inflammatory effect can be seen as the result of mediated reactions between peptides and proteases involved in these processes, as with the histamine H1-type receptor blocking.

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Abbreviations

BPTI:

bovine pancreatic trypsin inhibitor

TNF-α:

tumor necrosis factor alpha

LPS:

lipopolysaccharide

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku 159, Vladivostok, 690022, Russia

    O. V. Sintsova, E. A. Pislyagin, I. N. Gladkikh, M. M. Monastyrnaya, E. S. Menchinskaya, E. V. Leychenko, D. L. Aminin & E. P. Kozlovskaya

  2. School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia

    E. V. Leychenko

Authors
  1. O. V. Sintsova
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  2. E. A. Pislyagin
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  3. I. N. Gladkikh
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  4. M. M. Monastyrnaya
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  5. E. S. Menchinskaya
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  6. E. V. Leychenko
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  7. D. L. Aminin
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  8. E. P. Kozlovskaya
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Corresponding author

Correspondence to E. V. Leychenko.

Additional information

Original Russian Text © O.V. Sintsova, E.A. Pislyagin, I.N. Gladkikh, M.M. Monastyrnaya, E.S. Menchinskaya, E.V. Leychenko, D.L. Aminin, E.P. Kozlovskaya, 2017, published in Bioorganicheskaya Khimiya, 2017, Vol. 43, No. 1, pp. 105–112.

The paper is based on the materials of the “Chemical Biology 2016” conference; Novosibirsk, Russia, July 24–29, 2016.

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Sintsova, O.V., Pislyagin, E.A., Gladkikh, I.N. et al. Kunitz-type peptides of the sea anemone Heteractis crispa: Potential anti-inflammatory compounds. Russ J Bioorg Chem 43, 91–97 (2017). https://doi.org/10.1134/S1068162016060121

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  • DOI: https://doi.org/10.1134/S1068162016060121

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