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Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction

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Abstract

A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We demonstrated that fluorescent probes with the TMB fluorophore can be used in qPCR experiments.

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Abbreviations

FAM:

fluorescein

BODIPY:

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

TMB:

1,3,5,7-tetramethyl-BODIPY

TBTA:

tris(N-benzyltriazolylmethyl)amine

FRET:

Férster resonance energy transfer

qPCR:

quantitative PCR

qRT-PCR:

quantitative reverse transcription PCR

References

  1. Ryazantsev, D.Y., Tsybulsky, D.A., Prokhorenko, I.A., Kvach, M.V., Martynenko, Y.V., Philipchenko, P.M., Shmanai, V.V., Korshun, V.A., and Zavriev, S.K., Anal. Bioanal. Chem., 2012, vol. 404, pp. 59–68.

    Article  CAS  PubMed  Google Scholar 

  2. Horváth, P., Sebej, P., Solomek, T., and Kán, P., Org. Chem., 2015, vol. 80, pp. 1299–1311.

    Article  Google Scholar 

  3. Yang, M., Jalloh, A.S., Wei, W., Zhao, J., Wu, P., and Chen, P.R., Nat. Commun., 2014, vol. 5, p. 4981.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Yu, M., Ast, S., Yu, Q., Lo, A.T., Flehr, R., Todd, M.H., and Rutledge, P.J., PLoS One, 2014, vol. 9, p. e100761. doi: 10.1371/journal.pone.0100761

  5. Boldyrev, I.A. and Molotkovsky, Yul.G., Russ. J. Bioorg. Chem., 2006, vol. 32, pp. 78–83.

    Article  CAS  Google Scholar 

  6. Ustinov, A.V., Stepanova, I.A., Dubnyakova, V.V., Zatsepin, T.S., Nozhevnikova, E.V., and Korshun, V.A., Russ. J. Bioorg. Chem., 2010, vol. 36, pp. 401–445.

    Article  CAS  Google Scholar 

  7. Suzuki, T., Higgins, P.J., and Crawford, D.R., BioTechniques, 2000, vol. 2, pp. 332–337.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    E. V. Ilnitskaya, V. I. Martynov & A. A. Pakhomov

  2. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, 11991, Russia

    Yu. N. Kononevich & A. M. Muzafarov

  3. Enikolopov Institute of Synthetic Polymeric Materials, Russian Academy of Sciences, Moscow, 117393, Russia

    A. M. Muzafarov

  4. Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119899, Russia

    S. A. Rzhevskiy, I. A. Shadrin & E. V. Babaev

Authors
  1. E. V. Ilnitskaya
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  2. Yu. N. Kononevich
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  3. A. M. Muzafarov
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  4. S. A. Rzhevskiy
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  5. I. A. Shadrin
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  6. E. V. Babaev
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  7. V. I. Martynov
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  8. A. A. Pakhomov
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Corresponding author

Correspondence to A. A. Pakhomov.

Additional information

Original Russian Text © E.V. Ilnitskaya, Yu.N. Kononevich, A.M. Muzafarov, S.A. Rzhevskiy, I.A. Shadrin, E.V. Babaev, V.I. Martynov, A.A. Pakhomov, 2015, published in Bioorganicheskaya Khimiya, 2015, Vol. 41, No. 4, pp. 505–508.

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Ilnitskaya, E.V., Kononevich, Y.N., Muzafarov, A.M. et al. Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction. Russ J Bioorg Chem 41, 451–453 (2015). https://doi.org/10.1134/S1068162015040068

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  • DOI: https://doi.org/10.1134/S1068162015040068

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