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Determination of Free and Esterified Fatty Acids in Hydrocoles of Different Content of Polyunsaturated Fatty Acids by Gas–liquid Chromatography

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In a word, I have become cognisant of many, many things…

but still I am not yet satisfied. My spirit still thirsts for further knowledge…

St. John of Kronstadt

Abstract

This paper presents a new technique of hydrocole (bacteria, blue−green algae, phytoplankton, zooplankton, sponges, fish oil and seal fat) fatty acids (FA) methyl esters determination by gas-liquid chromatography. The polyunsaturated fatty acids (PUFA) contents in these objects were different. The distinguishing features of the technique are the next ones: efficiency, lipid extraction recovery ≥94% using the sonication during 10–15 min without sample drying, high PUFAs yield (≥97%) by reduction of oxidation up to 40% during methylation; exhaustive extraction of fatty acid methyl esters (FAMEs) including PUFA; a new approach to obtain of methyl esters of free fatty acids except using toxic reagents; accuracy of FAMEs quantification including ω-3-, ω-6-, ω-9-FA using the di-n-decyl ether as an internal standard and accuracy of measurements using “T14165QC Fish oil” standard sample (first). The technique is approbated for Baikal hydrocole FA determination from 20 to 2000 μg in the sample and might be used for scientific and applied tasks. The interlaboratory precision of FAME determination of phytoplankton and bacteria is ≤10%.

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ACKNOWLEDGMENTS

The authors thank the Head of the Laboratory of Hydrochemistry and Chemistry of the Atmosphere LIN SB RAS Doctor of geographical sciences Prof. T.V. Khodzher and PhD senior research I.I. Marinaite for GC-2010 Plus (Shimadzu) and chemicals kindly assigned, thank the academic of Russian Academy of Sciences Doctor of chemical sciences lead scientific worker Prof. M.A. Grachev and Doctor of biological sciences the Head of the Department of Ultrastructure of the Cell LIN SB RAS Prof. E.V. Likhoshvai for the equipment of Instrumental Center of Physical-Chemical Ultramicroanalysis of Collective Use of the LIN SB RAS kindly assigned. The authors express special thanks to the Head of the Laboratory of Chromatography LIN SB RAS PhD Docent Gorshkov A.G. for priceless help.

Funding

The investigation was carried out in the context of the federal task of the Ministry of the Science and High Education of the Russian Federation (projects of the Federal Agency of Science and Education no. 0345-2018-0001 “The investigation of evolution, ecological and molecular-biological aspects of silica-dependent chromista…”, no. 0345-2018-0008 “The estimation and prognosis of ecological status of Lake Baikal and adjacent territories…”, no. 0345-2018-0001 “Microorganisms of the deep-water biosphere…”, no. 0345-2018-0003 “Microbial and viral communities in biofilms of freshwater ecosystems…”) with the use of equipment of Instrumental Center of Physical-Chemical Ultramicroanalysis of Collective Use of the Limnological Institute of SB RAS (LIN SB RAS).

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  1. The Limnological Institute of The Siberian Branch of The Russian Academy of Sciences, 664033, Irkutsk, Russia

    A. A. Nikonova, S. M. Shishlyannikov, T. A. Shishlyannikova, T. N. Avezova, T. A. Babenko, O. I. Belykh, O. Yu. Glyzina, V. A. Obolkin, O. N. Pavlova, E. V. Sukhanova, I. V. Tikhonova & T. A. Khanaeva

  2. Institute of the Limnology of Russian Academy of Sciences, 196105, St. Petersburg, Russia

    S. M. Shishlyannikov

  3. The Irkutsk State University, 664033, Irkutsk, Russia

    A. N. Smagunova & V. A. Khutoryansky

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  1. A. A. Nikonova
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Nikonova, A.A., Shishlyannikov, S.M., Shishlyannikova, T.A. et al. Determination of Free and Esterified Fatty Acids in Hydrocoles of Different Content of Polyunsaturated Fatty Acids by Gas–liquid Chromatography. J Anal Chem 75, 1310–1321 (2020). https://doi.org/10.1134/S1061934820100093

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